To develop a HPIX-UV-MS method for identifying the constituents in theChinese drug Notoginseng (the root of Panax notoginseng). Methods A Phenomenex Luna C_(18) column(250 mm x 4.6 mm ID, 5 μm) was utilized. Water co...To develop a HPIX-UV-MS method for identifying the constituents in theChinese drug Notoginseng (the root of Panax notoginseng). Methods A Phenomenex Luna C_(18) column(250 mm x 4.6 mm ID, 5 μm) was utilized. Water containing 0.005% formic acid (A) and acetonitrilecontaining 0.005% formic acid (B) were used as gradient eluents. UV spectra were recorded in range195 - 400 nm. Both positive and negative ion ESI modes were used. Results The constituents inNotoginseng were well separated and detected. Fourteen compounds were identified by comparing theirretention time and ESI-MS data with those obtained from the reference compounds. Forty-one compoundswere deduced by data analysis of MS and literature; among them, yesanchinosides-H and -E,chikusetsusaponin-L_5, malonyl-ginsenoside-R_(g_1), the isomers of notoginsenosides-J, -A, -R_1, -G,-R_2, and ginsenoside-Rh_3 were discovered in Notoginseng for the first time. Conclusion Thismethod gives high sensitivity and good separation, and is suitable for identifying the constituentsin Notoginseng. This result is helpful for further phytochemical research on Notoginseng. Based onthis result, further quality control can be studied.展开更多
目的：采用高效液相色谱法检测发酵液中的替考拉宁含薰。方法：色谱柱为Discovery R C18流动相为乙腈-0．02mol／L乙酸铵，梯度洗脱，柱温30℃，检测波长为277nm。结果：替考拉宁的5个主要成分T-A2-1，T-A2-2，T-A2-3，T—A2—4和T—A2...目的：采用高效液相色谱法检测发酵液中的替考拉宁含薰。方法：色谱柱为Discovery R C18流动相为乙腈-0．02mol／L乙酸铵，梯度洗脱，柱温30℃，检测波长为277nm。结果：替考拉宁的5个主要成分T-A2-1，T-A2-2，T-A2-3，T—A2—4和T—A2—5在0．2-1．0mg／ml浓度范围内线性关系良好，相关系数（r）分别为0．9996、0．9992、0．9990、0．9994和0．9993。结论：该方法具有测定方便、快捷、结果准确等特点。展开更多
基金NationalBasicResearchProgramofChina (No .G19990 5 44 0 6)NationalNaturalScienceFoundationofChina(No .3 9970 898)
文摘To develop a HPIX-UV-MS method for identifying the constituents in theChinese drug Notoginseng (the root of Panax notoginseng). Methods A Phenomenex Luna C_(18) column(250 mm x 4.6 mm ID, 5 μm) was utilized. Water containing 0.005% formic acid (A) and acetonitrilecontaining 0.005% formic acid (B) were used as gradient eluents. UV spectra were recorded in range195 - 400 nm. Both positive and negative ion ESI modes were used. Results The constituents inNotoginseng were well separated and detected. Fourteen compounds were identified by comparing theirretention time and ESI-MS data with those obtained from the reference compounds. Forty-one compoundswere deduced by data analysis of MS and literature; among them, yesanchinosides-H and -E,chikusetsusaponin-L_5, malonyl-ginsenoside-R_(g_1), the isomers of notoginsenosides-J, -A, -R_1, -G,-R_2, and ginsenoside-Rh_3 were discovered in Notoginseng for the first time. Conclusion Thismethod gives high sensitivity and good separation, and is suitable for identifying the constituentsin Notoginseng. This result is helpful for further phytochemical research on Notoginseng. Based onthis result, further quality control can be studied.
文摘目的：采用高效液相色谱法检测发酵液中的替考拉宁含薰。方法：色谱柱为Discovery R C18流动相为乙腈-0．02mol／L乙酸铵，梯度洗脱，柱温30℃，检测波长为277nm。结果：替考拉宁的5个主要成分T-A2-1，T-A2-2，T-A2-3，T—A2—4和T—A2—5在0．2-1．0mg／ml浓度范围内线性关系良好，相关系数（r）分别为0．9996、0．9992、0．9990、0．9994和0．9993。结论：该方法具有测定方便、快捷、结果准确等特点。
