目的基于Notch信号通路探讨芦丁对结肠癌细胞增殖的影响。方法采用免疫组化技术对比人结肠癌组织及癌旁正常组织中Notch-1蛋白的表达情况;采用不同浓度芦丁作用于SW480细胞,检测其对结肠癌细胞增殖及Notch-1、Hes-1表达水平的影响。结果...目的基于Notch信号通路探讨芦丁对结肠癌细胞增殖的影响。方法采用免疫组化技术对比人结肠癌组织及癌旁正常组织中Notch-1蛋白的表达情况;采用不同浓度芦丁作用于SW480细胞,检测其对结肠癌细胞增殖及Notch-1、Hes-1表达水平的影响。结果与0μmol/L比较,芦丁0、20、40μmol/L浓度组Notch-1、Hes-1 m RNA及蛋白表达水平显著降低(均P<0. 01)。结论人结肠癌组织中Notch-1的表达明显增加,芦丁可以显著抑制SW480细胞的增殖,显著下调SW480细胞中Notch-1、Hes-1的m RNA和蛋白表达水平。展开更多
Urotensin-Ⅱ is the most potent vasoconstrictor peptide ever identified.A great number of studies about hemodynamics of urotensin-Ⅱ were reported.Recently,many researches have demonstrated that urotensin-Ⅱ also has ...Urotensin-Ⅱ is the most potent vasoconstrictor peptide ever identified.A great number of studies about hemodynamics of urotensin-Ⅱ were reported.Recently,many researches have demonstrated that urotensin-Ⅱ also has nonhemodynamic effects,such as mitogenic action,stimulating cell proliferation,promoting extracellular matrix production and so on.Nonhemodynamic effects of urotensin-Ⅱ may play a role in the pathogenesis of some diseases.展开更多
文摘目的基于Notch信号通路探讨芦丁对结肠癌细胞增殖的影响。方法采用免疫组化技术对比人结肠癌组织及癌旁正常组织中Notch-1蛋白的表达情况;采用不同浓度芦丁作用于SW480细胞,检测其对结肠癌细胞增殖及Notch-1、Hes-1表达水平的影响。结果与0μmol/L比较,芦丁0、20、40μmol/L浓度组Notch-1、Hes-1 m RNA及蛋白表达水平显著降低(均P<0. 01)。结论人结肠癌组织中Notch-1的表达明显增加,芦丁可以显著抑制SW480细胞的增殖,显著下调SW480细胞中Notch-1、Hes-1的m RNA和蛋白表达水平。
文摘Urotensin-Ⅱ is the most potent vasoconstrictor peptide ever identified.A great number of studies about hemodynamics of urotensin-Ⅱ were reported.Recently,many researches have demonstrated that urotensin-Ⅱ also has nonhemodynamic effects,such as mitogenic action,stimulating cell proliferation,promoting extracellular matrix production and so on.Nonhemodynamic effects of urotensin-Ⅱ may play a role in the pathogenesis of some diseases.
