摘要
磷酸化是生物体内广泛存在的一类蛋白质翻译后修饰,其参与了绝大多数生命活动的调控。鉴于磷酸化修饰有着重要的生物学意义,相关研究一直是不少科研工作者们关注的焦点。然而,因磷酸化蛋白具有分布广泛、相对丰度低、持续动态变化等特征,导致其分析过程存在复杂性高、效率低、难度大等问题。开发高效且稳定的样品前处理方法是实现高通量磷酸化修饰分析的关键因素之一。除基于金属氧化物亲和色谱法、固定化金属亲和色谱法的经典富集材料之外,近十年来,一些通过离子交换、氢键、配体交换、疏水作用等方式分离磷酸化多肽的新型材料也逐渐得到应用。本文简述了这些新兴富集方法的原理及研究进展。
Phosphorylation is a type of protein post-translational modification widely existing in organisms, and it is involved in the regulation of most life activities. In view of its important biological significance, the relevant research has been the focus of many scientific researchers. However, due to the characteristics of wide distribution, low relative abundance and continuous dynamic changes of phosphorylated proteins, the analysis process is extremely complicated, inefficient and difficult. It is one of the key factors to achieve high-throughput phosphorylation analysis via developing efficient and stable sample preparation methods. In the past decade, in addition to the classic enrichment materials based on metal oxide affinity chromatography and immobilized metal affinity chromatography, several novel ones based on ion exchange, hydrogen bonding, ligand exchange, and/ or hydrophobic interaction have been used to separate phosphopeptides. This review briefly describes the enrichment principles as well as the research progress of these emerging methods.
出处
《分析化学进展》
2021年第2期47-59,共13页
Advances in Analytical Chemistry
