摘要
蛋白质磷酸化修饰在细胞的信号转导、代谢、发育等生命过程中发挥着重要作用。除了研究较为透彻的发生在丝氨酸、苏氨酸和酪氨酸侧链羟基的O-磷酸化修饰之外,近年来,发生在组氨酸、精氨酸和赖氨酸侧链氨基的N-磷酸化修饰受到了越来越广泛的关注。然而,由于N-磷酸化修饰具有独特的P-N键结构,导致其化学稳定性差。尤其是在O-磷酸化肽段富集常用的酸性条件下,N-磷酸化极易丢失。因此,目前对N-磷酸化蛋白质的研究仍处于初始阶段。该文针对蛋白质N-磷酸化修饰的特点、富集和鉴定方法进行了综述,并对其发展前景进行了展望。
Protein phosphorylation plays an important role in various life processes,such as signaling,metabolism,and growth.In addition to the well-known O-phosphorylation,which occurs on serine,threonine and tyrosine side chains,N-phosphorylation,occurring on histidine,arginine and lysine side chains,has attracted much attention.However,due to the chemical instability of N-phosphorylation,the studies on it are still in the nascent stage.In this review,enrichment and detection methods for protein N-phosphorylation are reviewed,and future research in this field is prospected.
作者
胡晔晨
江波
张丽华
张玉奎
HU Yechen;JIANG Bo;ZHANG Lihua;ZHANG Yukui(CAS Key Laboratory of Separation Sciences for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China;University of Chinese Academy of Sciences,Beijing 100049,China)
出处
《色谱》
CAS
CSCD
北大核心
2020年第3期278-286,共9页
Chinese Journal of Chromatography
基金
国家自然科学基金(21505133,91753110)~~
