摘要
Objective: To evaluate the effects of the Syk mRNA expression in human breast cancer on tumor growth and metastasis,and the correlation of the Syk gene expression with ER,PR,P53,and HER2/neu. Methods:Using semi-RT-PCR,specimens from 40 breast cancer patients(tumor tissues,adjacent normal tissues),and 15 fibroadenoma were detected for the expression of the Syk gene and level of Syk mRNA.Meanwhile,ER,PR,P53,HER2/neu were detected in 40 tumor tissues from breast cancer with immunohistochemical staining. Results:Expression of the Syk gene was detected in all normal breast tissues.Unlike normal breast tissue,31 out of 40 breast cancer tissues did not show any detectable Syk mRNA expression,and there were significant differences in two groups(P<0.05).The level of Syk mRNA in the primary breast cancer tissues was significantly lower than that in the adjacent non-cancerous breast tissues and benign fibroadenoma breast tissues(P<0.05).Furthermore,only two breast cancer tissues in 18 patients with lymph node metastasis had the Syk mRNA expression.The Syk mRNA expression was negatively correlated to lymph node metastasis,HER2/neu protein expression(P<0.05). Conclusion: The expression of the Syk gene may play an important role in suppressing growth and metastasis of breast cancer.
Objective: To evaluate the effects of the Syk mRNA expression in human breast cancer on tumor growth and metastasis,and the correlation of the Syk gene expression with ER,PR,P53,and HER2/neu. Methods:Using semi-RT-PCR,specimens from 40 breast cancer patients(tumor tissues,adjacent normal tissues),and 15 fibroadenoma were detected for the expression of the Syk gene and level of Syk mRNA.Meanwhile,ER,PR,P53,HER2/neu were detected in 40 tumor tissues from breast cancer with immunohistochemical staining. Results:Expression of the Syk gene was detected in all normal breast tissues.Unlike normal breast tissue,31 out of 40 breast cancer tissues did not show any detectable Syk mRNA expression,and there were significant differences in two groups(P<0.05).The level of Syk mRNA in the primary breast cancer tissues was significantly lower than that in the adjacent non-cancerous breast tissues and benign fibroadenoma breast tissues(P<0.05).Furthermore,only two breast cancer tissues in 18 patients with lymph node metastasis had the Syk mRNA expression.The Syk mRNA expression was negatively correlated to lymph node metastasis,HER2/neu protein expression(P<0.05). Conclusion: The expression of the Syk gene may play an important role in suppressing growth and metastasis of breast cancer.
基金
SupportedbygrantfromtheScienceFundofDepartmentofScienceandTechnologyofJiangsuProvince(0 3KJD3 2 0 13 8)
