摘要
目的探讨IL-13,ET-1,甲基强的松龙及肝素钠对肾小球系膜细胞膜表达细胞周期负控p27的变化。方法不同浓度IL-13(10ng/ml,100ng/ml),ET-1(10-7mol/L),甲基强的松龙0.5μg/ml,肝素钠100mg/L和200mg/L,作用于培养的系膜细胞。流式细胞仪检测p27表达水平。结果IL-1310ng/ml组和100ng/ml浓度组p27表达为(46.74±3.25)和(23.8±2.56),与对照组比较有明显差异(P<0.001,P<0.05),两浓度组之间有显著差异(P<0.01)。ET-1刺激14h和18h后p27的表达分别为(14.76±1.49)和(12.18±1.30),与对照组比较均有明显差异(P<0.05,P<0.05)。甲基强的松龙48h及72hp27表达为(9.9±1.01)和(9.12±0.93),与对照组比较明显降低,P<0.001。肝素钠100mg/L和200mg/L浓度p27的表达分别为(26.94±1.23)和(28.04±0.99),较对照组明显升高(P<0.05),不同浓度组间比较无明显差异。结论肾小球系膜细胞受不同因素作用后细胞周期调节负控蛋白p27表达水平不同。p27在调节系膜细胞增殖过程中起重要作用。
Objective: To observe the changes of cyclin kinase inhibitor p27 expressed by glomerular mesangial cells in response to IL-13? ET-1? methylprednisolone and heparin sodium. Methods: IL-13(10 ng/ml and 100 ng/ml), ET-1(10-7 mol/L), methylprednisolone (0.5 μg/ml) and heparin sodium (100 mg/L, 200 mg/L) acted on cultured glomerular mesangial cells and p27 level was assessed by flow cytometry. Results: The levels of p27 in IL-13 groups were (46.74±3.25) 10 ng/ml and (23.8±2.56) 100 ng/ml, which were increased significantly compared with that of controls (P <0.001, P <0.05). Stimulating mesangial cells with ET-1 for 14 and 18 hours, the levels of P27 were lower than that of controls (P <0.05, P <0.05). Expressions of P27 with methylprednisolone for 48 and 72 hours were (9.90±9.12) and (9.12±0.93), was significantly lower than that of controls (P <0.01). Expressions of p27 in heparin sodium group (200 mg/L, 100 μg/L) were increased significantly compared with that of controls (P <0.05). Conclusions: Cyclin kinase inhibitor p27 is a very important factor in regulating glomerular mesangial cell proliferation.
出处
《中国现代医学杂志》
CAS
CSCD
2004年第10期98-101,共4页
China Journal of Modern Medicine
