摘要
目的 :明确牙周基础治疗与口腔幽门螺杆菌 (Helicobacterpylori ,Hp)存在的关系。分析胃Hp与口腔Hp的基因型 ,以明确胃与口腔的Hp是否具有同源性。 方法 :选择 5 6例有慢性中度牙周炎且胃镜活检Hp感染阳性患者分为 2组。提取所有口腔样本中的DNA ,PCR扩增 ,比较药物治疗组与药物治疗加牙周基础治疗组治疗前后 2组口腔Hp检出率的差别。对比分析其中 5位患者和 1位家属胃和口腔PCR产物的序列。结果 :2组患者不同的治疗后口腔Hp检出率有显著性差别 ( 3 .0 %vs 2 6.1% ,P <0 .0 5 )。胃和口腔Hp的序列相比较 ,相同者 1例 ,可能相同者 1例 ,有 1～ 4个碱基不同者 2例。 1例患者及其家属口腔中均检出Hp ,两者的序列有 3个不同。 结论 :牙周基础治疗有助于口腔Hp的根除。口腔中有可能同时存在多个Hp菌株 。
Objective: To investigate whether initial periodontal therapy may affect the presence of oral Helicobacter pylori(Hp), and to compare the genotype of Helicobacter pylori in oral cavity and that in stomach. Methods: 56 patients with gastric Hp and periodontitis were enrolled in this study. PCR was carried out to identify the presence of Hp in the samples before and after initial periodontal therapy(group 1) or medicine therapy(group 2). DNA sequence of PCR products of 5 patients and 1 Hp infected patient's relative was compared and analyzed. Results: Four weeks after medicine therapy, the rate of Hp in oral cavity was singnificantly lower in group 1 than that in group 2 (3.0% vs 26.1%, P<0.05). An identical sequence of oral and stomach Hp was found in 1 patient, most likely identical in 1 case, difference of 1～4 bases in 2 cases. In 1 case and his family oral Hp showed three different types of DNA sequences. Conclusion: There may be multi- Hp strain existing in oral cavity. Oral colonization of Hp may imply that there is a risk of the relapses of gastric and duodenal Hp infection.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2004年第3期353-357,共5页
Journal of Practical Stomatology
基金
国家自然科学基金 (30 0 0 0 1 92 )资助
关键词
幽门螺杆菌
牙周炎
牙菌斑
聚合酶链反应
基因型
Helicobacter pylori
Dental plaque
Periodontitis
Polymerase chain reaction
Genotype
