摘要
背景与目的:口腔鳞状细胞癌(oralsquamouscellcarcinoma,OSCC)中c-myc和p16的蛋白表达已有一些报道,其基因扩增和mRNA表达研究很少,未见有从多个水平探讨OSCC中c-myc和p16协同作用的研究。本研究旨在探讨OSCC中c-myc和p16基因在基因扩增、mRNA表达和蛋白质表达三个水平上的协同作用。方法:用原位杂交和免疫组化技术与图像分析相结合的方法,对30例OSCC组织中c-myc和p16两种基因的扩增、mRNA表达和蛋白表达状况进行定性、定位、定量研究。结果:OSCC中c-myc的扩增阳性率为63.3%,p16基因未见扩增。c-myc和p16在OSCC中的mRNA表达率分别是83.3%和93.3%,蛋白表达率分别是60.0%和86.7%。相关性分析表明,p16与c-myc在mRNA表达(r=0.6765,P=4.0556E-05)和蛋白表达(r=0.5642,P=0.0012)都呈显著性相关。结论:c-myc基因扩增和高表达在OSCC发生、发展中具有重要作用。在OSCC中c-myc和p16的表达具有一定的内在联系。
BACKGROUND &OBJECTIVE: The study on the protein expression of c-m yc and p16 in oral squamous cell carcinoma (OSCC) has been reported, and a few r esearches involved in their gene amplification and mRNA expression in OSCC, howe ver, the synergism of c-myc and p16 in OSCC in multiple levels is still unclear . This study aims to probe the synergism of c-myc and p16 in OSCC in gene ampli fication, mRNA expression, and protein expression levels. METHODS: The gene ampl ification, expression of mRNA, and protein of c-myc and p16 in 30 cases of OSCC were determined by in situ hybridization and immunohistochemical techniques. RE SULTS: The amplification rate of c-myc was 63.3%, but no amplification of p16 was found in OSCC. The mRNA expression rates of c-myc and p16 were 83.3%and 93 .3%, and their protein expression rates were 60.0%and 86.7%, respectively. Th e correlation analysis showed that there was significant correlativity between p 16 and c-myc both in mRNA expression (r=0.676 5, P=4.055 6E-05) and in protein expression (r=0.564 2, P=0.001 2). CONCLUSION: The gene amplification and overe xpression of c-myc plays an important role in the tumorigenesis and development of OSCC. There is certain internal relationship between p16 and c-myc expressi on in OSCC.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2004年第6期635-639,共5页
Chinese Journal of Cancer
基金
山东省卫生厅青年科研基金(No.1998CA2CFB1)
