摘要
用甲醇对 6种野生稻E6 1 ,E6 3/6 4 (大颖野生稻的不同生态型 ) ,E7 4 (颗粒野生稻 ) ,E9 1 0、E9 2 0 (阔叶野生稻的不同生态型 ) ,E1 3 1 3(小粒野生稻 ) ,E1 5 1 3(药用野生稻 )和E1 6 3(斑点野生稻 )进行了索氏提取 ,提取率分别为1 9 6 2 % ,1 5 6 0 % ,1 8 90 % ,8 5 0 % ,1 6 2 7% ,2 4 86 % ,1 0 0 4 %和 1 3 32 %。通过测定这 6种野生稻抽提物对 3龄斜纹夜蛾幼虫的选择性拒食活性和非选择性拒食活性 ,筛选出大颖野生稻抽提物E6 1对斜纹夜蛾有较强的拒食作用。用E6 1对叶碟处理后 ,在 1 %的浓度下 ,2 4h和 4 8h测得其对 3龄斜纹夜蛾选择性拒食率均为 1 0 0 % ,而非选择性拒食率分别为 4 0 95 %和 4 7 2 7% ;毒力测定表明 2龄斜纹夜蛾幼虫 2 4h和 4 8h后的拒食中浓度 (AFC50 )分别为 1 2 0mg/mL和 5 0mg/mL。
Six species of wild rice E6 1, E6 3/6 4 (different ecotypes of Oryza grandiglumis), E7 4(O. granulata), E9 10, E9 20(different ecotypes of O. latifolia),E13 13(O. minuta), E15 13 (O. officinalis) and E16 3(O. punctata) were extracted with methnol by Sohxlet method. The extract rates of the six species of wild rice were 19 62%,15 60%,18 90%,8 50%,16 27%, 24 86%,10 04% and 13 32%, respectively. Selective antifeedant activity and non selective antifeedant activity of extract from the six species of wild rice against the 3rd instar larvae of Spodoptera litura showed that the extract of the wild rice E6 1 was better than the others.When the concentration was 1%, selective antifeedant activity of the E6 1 extracts was 100% in 24 h and 48 h and non selective antifeedant activity was 40 95% and 47 27% in 24 h and 48 h after treatment, respectively.The concentration of 50% antifeedant of the wild rice E6 1 extract against 2nd instar larvae of Spodoptera litura was 120 mg/mL in 24 h and 50 mg/mL in 48 h after treatment.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2004年第4期371-373,共3页
Chinese Journal of Rice Science
基金
国家自然科学基金资助项目 ( 3 0 170 0 97)
广东省自然科学基金资助项目 ( 990 70 3 )
