摘要
以干扰作用控制指数 (IIPC)为评价指标 ,小菜蛾为试虫 ,分别对苦槛蓝茎叶的不同溶剂提取物进行了生物活性测定 .结果表明 ,石油醚和氯仿萃取部分具有较强的生物活性 ,而乙酸乙酯和乙醇萃取部分活性较低 .在 0 0 1gDW·ml-1时 ,用石油醚和氯仿萃取物对小菜蛾处理 1d后的产卵忌避率 (ODR)分别为84 6 9%、79 90 % ,3d后为 76 4 7%、4 5 70 % ,IIPC为 0 15 6 5和 0 2 0 5 5 .在 0 0 5gDW·ml-1·L-1时 ,1d后为 88 5 2 %和 72 2 5 % ,3d后为 87 33%和 5 8 37% ,IIPC为 0 112 5和 0 2 6 2 0 .进而对氯仿部分进行了柱层析分离 ,并鉴定了 3种黄酮类化合物 ,它们分别是 5 ,7 二羟基黄烷酮 (Ⅰ )、5 ,7 二羟基黄酮醇 (Ⅱ )、3,4’ 二甲氧基 5 ,7 二羟基黄酮醇 (Ⅲ ) ,其中Ⅱ在生测中表现出较好的活性 .
The bioactivity of Myopdrum bontioides extracts to Plutella xylostella was studied with IIPC as evaluated index.The results showed that petroleum ether and chloroform extracts had a higher activity than ethyl acetate and alcohol extracts.At 0.01gDW·ml -1,the ODR of petroleum ether and chloroform extracts was 84.69% and 79 90%,and 76.47% and 45.70% after treated 1d and 3d,while the IIPC was 0.1565 and 0.2055,respectively.Provided with a higher concentration of 0.05 gDW·ml -1,the ODR was 88.52% and 72.25%,and 87 33%,58.37%,while the IIPC was 0.1125 and 0.2620,respectively.From the chloroform extract of Myopdrum Bontioides,three flavonoids,2,3-dihydro-5,7-dihydroxy-2-phenyl-4H-1-Benzopyran-4-one(Ⅰ),3,5,7-trihydroxy-2-phenyl-4H-1-Benzopyran-4-one(Ⅱ)and 5,7-dihydroxy-3-methoxy-2-(4-methoxyphenyl)-4H-1-Benzopyran-4-one(Ⅲ),were isolated,and their structure were identified based on the analyses of physical and spectrum data.Among these compounds,(Ⅱ)had a better bioactivity to Plutella xylostella.
出处
《应用生态学报》
CAS
CSCD
2004年第7期1171-1173,共3页
Chinese Journal of Applied Ecology
基金
中国科学院上海有机研究所生命有机重点实验室基金 ( 2 0 0 1.7 2 0 0 2 .7)
华南农业大学校长基金资助项目 ( 4 90 0 K990 192 )
关键词
苦槛蓝
小菜蛾
生物活性
黄酮类化合物
Myoporum bontioides, Plutella xylostella, Bioactivity, Flavonoids.
