摘要
目的 :构建结核分枝杆菌Ag85B和MPT6 4编码基因的共表达载体 pBud85B -MPT。 方法 :将结核分枝杆菌Ag85B、MPT6 4基因同时克隆进多启动子共表达载体pBudCE4 .1中 ,得到真核共表达质粒 pBud85B -MPT ;将 pBud85B -MPT转染COS - 7细胞 ,通过RT -PCR方法检测目的基因的表达。结果 :在COS - 7细胞中同时检测到Ag85B、MPT6 4的表达。 结论 :pBud85B -MPT共表达质粒构建成功 ,为进一步研究新型结核病疫苗奠定基础。
Objective:To construct an eukaryotic coexpression plasmid containing Mycobacterium tuberculosis Ag85B and MPT64 gene.Methods:Mycobacterium tuberculosis Ag85B and MPT64 gene were cloned into pBudCE4.1 to construct recombinant plasmid pBud85B-MPT.The recombinant plasmid was transfected into COS-7 cells,and its expression of Ag85B and MPT64 was assesed by RT-PCR.Results:Mycobacterium tuberculosis Ag85B and MPT64 were detected in transfected COS-7 cells.Conclusion:The recombinant plasmid pBud85B-MPT can express Ag85B and MPT simultaneously in COS-7 cells which provides the basis for further investigation of DNA vaccine against tuberculosis.
出处
《重庆医科大学学报》
CAS
CSCD
2004年第3期276-278,共3页
Journal of Chongqing Medical University
基金
重庆市卫生局重点项目 ( 0 0 -10 0 6)
