摘要
目的分析结核分枝杆菌(Mycobacterium tuberculosis,MTB)mpt64基因突变情况。方法提取MTB标准株H37Rv和45株MTB临床株的DNA,扩增mpt64基因并进行序列分析。结果 MTB标准株H37Rv和44株MTB临床株的mpt64基因无突变,1株MTB临床分离株的mpt64基因存在63个碱基的缺失突变。结论结核分枝杆菌mpt64基因突变频率低。
Objective To investigate the mpt64 gene mutation situation of Mycobacterium tuberculosis（MTB）. Methods DNA extraction was performed from reference strain H37Rv and 45 clinical MTB isolation strains. Then the amplification and sequencing of mpt64 gene were accomplished. Results No mution of mpt64 gene was found in H37Rv and clinical isolation strains except for one clinical MTB strain had a deletion of 63 nucleotides. Conclusion The mutation prevalence of mpt64 gene of MTB is low.
出处
《实验与检验医学》
CAS
2016年第2期137-139,共3页
Experimental and Laboratory Medicine
基金
广东省省级科技计划项目(2014A020212173)
南方医科大学科研启动计划项目(C1031780)
