摘要
目的:观察不同培养的条件下兔骨髓间充质细胞的成骨特性。方法:比较骨髓间充质细胞在普通培养基培养、诱导培养基培养和新鲜兔骨碎粒的共培养3种条件下的成骨分化特性,观察3种条件下细胞的形态及碱性磷酸酶(ALP)活性、骨钙素水平,并用免疫组化方法检测Ⅰ型胶原、骨钙素。结果:共培养及诱导培养的间充质细胞同期ALP活性及细胞内骨钙素均显著高于普通培养组(P<0.01),诱导培养最高;经过诱导培养及共培养的间充质细胞,其Ⅰ型胶原、骨钙素免疫组化阳性;对照组Ⅰ型胶原免疫组化弱阳性、骨钙素免疫组化阴性。结论:诱导培养及共培养条件呈现促间充质细胞向成骨方向转化的特点,诱导培养短期能使ALP、骨钙素达到较高水平。
AIM:To investigate osteogenic characteristics of rabbit bone marrow mesenchymal stem cells (BMSC) under different culture conditions. METHODS:Osteogenesis differentiation characteristic of BMSC were compared in three different conditions: ordinary culture medium (DMEM/F12), induced medium and co culture with fresh bone particles. It was to observe the form of cells, alkaline phosphatase (ALP) activity and osteocalcin level. Meanwhile, collagen Ⅰand osteocalcin were assayed with immunocytochemical method. RESULTS:Corresponding ALP activity and osteocalcin level of mesenchymal cells, which were co cultured and induced cultured, were significantly higher than that in ordinary cultured group (P< 0.01), with induction cultured the highest; immunohistochemistry of collagen Ⅰand osteocalcin was positive with induced culture and co culture; while negative in control group. CONCLUSION:Induced culture and co cultured can promote the transformation of BMSC into osteoblasts, and make ALP activity and osteocalcin to a high level through a short period of induced culture.
出处
《中国临床康复》
CSCD
2004年第17期3261-3263,i002,共4页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金资助项目(39830100)
全军十五医药卫生科研重点基金资助项目(01Z072)~~
