摘要
利用在纤维蛋白平板法基础上进行改造的纤维蛋白原—琼脂糖平板法,筛选具有纳豆激酶活性的基因工程酵母菌。并用纤维蛋白平板法测定纳豆激酶纤溶活性。通过甲醇诱导从近100个菌落中筛选出5株纳豆激酶活性较高的重组纳豆激酶工程菌。同时确定了重组纳豆激酶酵母菌的最佳甲醇诱导浓度2%,产酶高峰时间为96h。
Gene engineering saccharomycete with nattokinase activity are screened by using modified fibrin-agarose plate method. Its fibrinolysis activity is detected by fibrin plate method. Five strains, within one hundred of them, with higher fibrinolysis are screened successfully by methanol inducing. Optimum methanol concentration of the P. pastoris recombinant engineering saccharomycete is 2% and the period of time for activity peaks is 96 h.
出处
《大连轻工业学院学报》
2004年第2期100-103,共4页
Journal of Dalian Institute of Light Industry
