摘要
目的:了解HCAP1蛋白在急性白血病患者外周血原始细胞中的表达。方法:分离18个正常个体外周血白细胞和20例急性白血病患者原代白血病细胞,用免疫细胞化学染色和Western印迹分析HCAP1蛋白表达并进行比较。结果:受检的正常入外周血自细胞和白血病患者原始细胞均表达HCAP1蛋白;在正常人外周血成熟白细胞,HCAP1主要分布于细胞核,而急性白血病患者的原始细胞在细胞核和细胞质均有分布;急性白血病患者原始细胞HCAP1蛋白的相对表达值(HCAP1／actin的灰度比值)为(0.37±0.23),正常入外周血白细胞的HCAP1蛋白相对表达值为(0.31±0.19,P>0.05)。结论:正常人外周血白细胞和白血病患者的原代白血病细胞均表达HCAP1蛋白,两者之间表达水平并无显著差异,但其在细胞内的分布有所不同。
Objective: To investigate the expression of hepatocellular carcinoma-associated protein 1(HCAP1) in the leukemic cells and normal peripheral white blood cells. Methods: The expression of HCAPl in the normal peripheral white blood cells of 18 healthy volunteers and in the leukemic cells of 20 patients with acute leukemia was examined using immuno-cytochemical staining and Western blot assay. Results: HCAPl was detected in both normal peripheral white blood cells and leukemic cells. Immunocytochemical staining showed that HCAPl localized in the nuclear in normal peripheral white blood cells and in the cytoplasm and nuclear in the leukemic cells. Western blot assay showed that the ratio of the HCAP1 and actin (HCAP1/actin, 0. 37 ± 0. 23) in leukemic cells was similar to that in the normal peripheral white blood cells(0. 31 ± 0. 19, P>0. 05). Conclusion: The HCAPl can be expressed in both normal peripheral white blood cells and the leukemic cells, and there is no difference in the protein levels between them.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2004年第5期512-514,共3页
Academic Journal of Second Military Medical University
基金
广东省自然科学基金(010411)教育部重点科学基金(教技司[2000]156号)广东省医学科研基金(A2001309).
