摘要
目的研究甲状旁腺素相关肽(PTHrP)对骨髓基质细胞RANKL/OPG基因表达的影响。方法分离小鼠骨髓细胞后,45 ng/ml的PTHrP刺激,第6天时观察破骨细胞生成情况。90 ng/ml PTHrP刺激贴壁鼠骨髓基质细胞3 d和6 d,实时荧光PCR检测破骨细胞分化因子RANKL及骨保护素OPG基因的表达情况。结果在PTHrP的刺激下,大量破骨细胞生成。骨髓基质细胞在PTHrP的刺激下,RANKL基因表达升高,OPG基因表达降低。结论PTHrP刺激破骨细胞生成是通过上调RANKL基因表达,下调OPG基因的表达实现的。
Objective To study the OPG/RANKL gene expression of bone marrow stromal cell under stimulation of PTHrP.Method Primary bone marrow cells were cultured under the stimulation of 45 ng/ml PTHrP. Number of osteoclasts was counted after 6-day's culture.90 ng/ml PTHrP was used to stimulate adherent bone marrow stromal cells for 3 or 6 days.OPG and RANKL gene expression level were determined by real-time PCR. Result Large number of osteoclasts were formed in primary bone marrow cell culture after 6-day stimulation of PTHrP.RANKL gene expression level was up-regulated by PTHrP,while OPG gene expression level were down-regulated.Conclusion PTHrP stimulates osteoclast formation via up-regulating RANKL level and down-regulating OPG level.
出处
《中华风湿病学杂志》
CAS
CSCD
2004年第2期77-79,i001,共4页
Chinese Journal of Rheumatology
