摘要
应用聚丙烯酰胺凝胶电泳技术研究了牙鲆养殖群体(F1代)的遗传结构.结果表明,牙鲆养殖群体具有较高的遗传多样性水平.牙鲆群体平均每位点等位基因数的变化为1 19(0 11)～1 24(0 10).多态位点百分数的变化为14 29%～23 81%.期望杂合度的变化为0 056(0 036)～0 057(0 033).观察杂合度的变化为0 099(0 065)～0 104(0 065).牙鲆群体间遗传分化较低,群体间分化度为0 005,表明总的遗传变异中有99 5%来自群体内,有0 5%来自群体间.群体间遗传距离和遗传一致度均值分别为0 001和0 999.群体间基因流很大,等于49 75.
Genetic structure of cultivated stocks was investigated using the assay of vertical slab polyacrylamide gel electrophoresis in Paralichthys olivaceus. Higher level of genetic diversity in the stocks was found: Mean number of alleles per locus(A) is 1.19 (0.11)～1.24 (0.10), percentage of polymorphic loci (P_(0.99)) is 14.29%～23.81%, the observed heterozygosity(H_o) is 0.099 (0.065)～0.104 (0.065),expected heterozygosity (H_e) is 0.056 ((0.036))～0.057 (0.033). The results showed that the coefficient of gene differentiation between the stocks is low, F_(st) =0.005, there is no significant difference in biochemical genetic characteristics among the stocks. The genetic distance and genetic identity is 0.001 and 0.999 respectively. Gene flow among the stocks is large (Nm=(49.75)).
出处
《海洋学报》
CAS
CSCD
北大核心
2004年第3期102-108,共7页
基金
福建省重点科技攻关资助项目(2002N020).
关键词
牙鲆
等位酶
遗传结构
遗传多样性
遗传分化
Paralichthys olivaceus
allozyme
genetic structure
genetic diversity
genetic differentiation
