摘要
用LKB水平电泳仪,4.4%聚丙烯酰胺凝胶,对10—11月龄团头鲂(全长14—16cm)的11种组织——白肌、晶体、脑、脾、肾、性腺、心、肝、鳍、红血球及血清的乳酸脱氢酶和苹果酸脱氢酶作电泳,再用LKB激光光密度计对同工酶的迁移率及活性作定性定量分析。在不同组织中,同工酶的迁移率和活性有明显的组织特异性。
In LKB electrophoresis set with 4.4% polycrylamide gel, the lactate dehydrogenase(LDH) and malate dehydrogenase (MDH) were examined from 11 tissues-white muscle, eye lens,brain, liver, kidney, gonad, heart, spleen, fin, red cell and serum of blunt snout bream. Qualitativeand quantitative analysis on mobility and activity were conducted by a LKB Ultorscan laser de-nsitometer. The activity and mobility of isozymes in different tissues showed a significant tissuespecificity.
出处
《上海水产大学学报》
CSCD
1992年第1期38-47,共10页
Journal of Shanghai Fisheries University
基金
加拿大国际发展研究中心组织和资助的亚洲鱼类遗传研究网的中国课题~~
