摘要
①目的 研究人发角蛋白非神经移植材料的解剖学特点和组织相容性 ,及其对周围神经缺损的修复效果。②方法 Wistar大白鼠 18只随机分成 3组 ,将其坐骨神经切取 10mm ,分别用人发角蛋白非神经移植材料(实验组 )、大白鼠自体骨骼肌 (对照Ⅰ组 )和未经特殊处理的人发 (对照Ⅱ组 )连接神经两断端。术后不同时间进行组织形态及解剖学观察。③结果 术后第 8周实验组坐骨神经两断端之间出现白色新生组织 ;第 12周白色新生组织出现于移植材料腔隙 ;第 2 4周移植材料腔隙被充满 ,人发被初步降解 ,光镜下可见人发周围有大量呈无序排列的再生神经纤维 ,透射电镜下可见人发周围雪旺细胞增殖并形成髓鞘。对照组未出现上述变化。④结论 人发角蛋白非神经移植材料的组织相容性好 ,并可诱导神经纤维再生 。
Objective To study the anatomy and histocompatibility of non-nerve grafts of the human-hair keratin, and to observe its effects on the repair of peripheral nerves. Methods Eighteen Wistar rats were divided into three groups. The sciatic nerve,10 mm long, of the rat was removed and transplanted with human-hair keratin graft, skeletal muscle and untreated hair, respectively. The characteristics of histomorphology and anatomy were observed at different times after the surgery. Results White tissues appeared between the broken ends of the sciatic nerve at the 8th post-operative week in the experimental group, and in human-hair keratin at the 12th week. At the 24th week large amount of infantile myelinated nerve fibers were observed under optical microscope regenerating around the human-hair, which was partially degraded and absorbed. Schwanns cells were observed under an electron microscope and medullation formed. Conclusion The non-nerve grafts of the human-hair keratin are well compatible with the body tissues, and could induce nerve regeneration, are ideal for the repair of nerve injuries.
出处
《齐鲁医学杂志》
2004年第2期102-104,共3页
Medical Journal of Qilu
