摘要
以皖 8 2 - 1 78(抗 )×通山薄皮黄豆甲 (感 )组合衍生的重组近交系群体为材料 ,以幼虫重为抗性鉴定指标 ,对大豆抗斜纹夜蛾的基因 (QTL)进行SSR分子标记研究。从大豆公共连锁图谱(Creganetal.,1 999) 7个与抗虫性可能有关的连锁群上选取了 1 0 3对SSR引物 ,鉴定亲本和RIL家系DNA ,其中 1 8对引物在RIL家系间表现出多态性。应用t测验和方差分析法分析SSR标记与性状的连锁 ,并以R2 表示标记对性状变异的解释率。结果在D2、C2、H连锁群上分别找到一个与大豆对斜纹夜蛾抗性有关的SSR标记 ,即Satt1 35、Satt36 3、Satt4 42 ,单个标记对抗性变异的解释率分别为 5 .5 7%、2 .89%、8.7%。
There have been few reports about molecular marker analysis of resistance to insect in soybean. This study was to determine quantitative trait loci (QTLs) associated with the soybean resistance to insect (Cotton Worm) by estimating linkage to simple sequence repeat (SSR) markers. Recombinant inbred lines (RILs) constructed through a cross between Wan82-178 and Tongshanbopihuangdoujia was used. Larval weight was examined in as index of resistance. Using the intergrated genetic linkage map of soybean (Cregan et al. 1999), we chose 103 SSR markers scattering on 7 linkage groups (LGs) that had the potential to map resistant QTL to screen polymorphic markers between Wan82-178 and Tongshanbopihuangdoujia. It was found that 18 SSR primers showed polymorphic DNA bands among these RIL lines. With t-test analysis and analysis of variance, three markers (Satt135,Satt363,Satt434) were found linked with the resistance genes, situating on three LGs, i.e. D2, C2, and H. The individual effect of each marker was reported as R 2 value, i.e 5.57%, 2.89%, and 8.7% respectively.
出处
《大豆科学》
CAS
CSCD
北大核心
2004年第2期91-95,共5页
Soybean Science
基金
973课题 (G19980 10 2 0 6)
863课题 ( 2 0 0 2AA2 110 5 2 )
关键词
大豆
斜纹夜蛾
抗生性
基因
SSR
微卫星标记
性状
变异
Soybean(Glycine max(L.) Merrill)
Cotton Worm( Prodenia litura (L.)Fabricius )
Antibiosis to insect
SSR
