摘要
目的 建立生物检材中五氯酚的高效液相色谱检测方法。方法 将鱼内脏组织匀浆 ,用有机溶剂萃取后 ,采用高效液相色谱仪检测。对高效液相色谱条件进行反复筛选 ,同时以空白添加实验考察回收率、线性范围、检测限。结果 建立了以乙醚为提取溶剂处理样品 ,采用 Hypersil ODS柱 (4 .6 m m i.d.× 15 0 m m,5μm) ,甲醇∶2 g/ L醋酸铵水溶液 (80∶ 2 0 )为流动相的高效液相色谱分析方法。30 5 nm进行检测 ,同时收集全光谱信息。按上述方法分析生物检材中的五氯酚 ,方法平均回收率为 76 % ,检测线性范围为 0 .0 4 2μg/ g～ 1.344μg/ g,相关性良好 (r=0 .9994 ) ,检测限为 0 .0 14μg/ g。运用该方法成功地从中毒鱼体中检出五氯酚。结论 所建立方法能便捷、准确地检测生物检材中五氯酚 ,为法医学中毒鉴定提供了新方法。
Objective To develop a method of detecting pentachlorophenol in biomaterial. Methods The samples were extracted by ether, and the characters of the high performance liquid chromatography (HPLC) method were studied. Results A method for the detection of pentachlorophenol in fish tissue samples was set up with the conditions of chromatography as follows: the compounds are separated on Hypersil ODS column(4.6 mm i.d.×150 mm,5 μm) and eluted with methanol which contains 2 g/L NH 4Ac(20∶80) using 305 nm as the detecting wavelength, the recovery of method being 76%, the linear range 0.042 μg/g^1.344 μg/g, and the correlation coefficient 0.9994, the limit of quantification 0.014 μg/g. Finally, pentachlorophenol was successfully detected in the fish tissue samples. Conclusion The method has been proven useful in the forensic cases involved in the poisoning of pentachlorophenol.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2004年第3期427-428,共2页
Journal of Sichuan University(Medical Sciences)
