摘要
目的 分析血浆游离爱泼斯坦 巴尔病毒 (Epstein Barrvirus,EBV)DNA定量测定在鼻咽癌诊治中的临床应用价值。方法 采用荧光定量聚合酶链反应 (fluorescencequantitativepolymerasechainreaction ,FQ PCR)技术 ,测定 6 6份治疗前或治疗后的鼻咽癌患者血浆EBV DNA含量 ,并与 30例健康对照者相比较。分析该含量与鼻咽癌分期、淋巴结转移灶负荷以及治疗后短期疗效之间的相关性。结果 鼻咽癌患者血浆EBV DNA阳性率 (6 6 7% ,2 8/ 4 2例 )和复制 (中位数 5 2 2 0拷贝 /ml)均明显高于健康对照者 (阳性率 10 % ,3/ 30例 ;复制中位数 0 ) ;治疗前组中 ,Ⅲ～Ⅳ期患者复制量 (中位数3130 0拷贝 /ml)高于Ⅰ～Ⅱ期 (中位数 4 0 0 0拷贝 /ml) ,N2 3患者 (中位数 32 5 0 0拷贝 /ml)高于N0 1(中位数 4 0 0 0拷贝 /ml)。而且 ,治疗后患者 (中位数 0 )低于治疗前 (中位数 5 2 2 0拷贝 /ml)。治疗后短期疗效评估发现 ,病灶有残留者DNA含量 (中位数 2 0 83 0拷贝 /ml)高于病灶消退良好者 (中位数0 )。结论 血浆游离EBV
Objective To elucidate the clinical value of quantitative analysis of plasma cell free EBV DNA in patients with nasopharyngeal carcinoma(NPC) Methods Using fluorescence quantitative polymerase chain reaction (FQ PCR), the copies of EBV DNA in 66 plasma samples of pre and post treated NPC patients were examined, and compared with 30 normal controls The relationships between the copies of free EBV DNA and tumor stage, metastases load, as well as short term therapeutic response were analyzed Results The positive percentage and copies of plasma EBV DNA of NPC patients(66 7%,Median 522 0 )were significantly higher than those of normal controls(10 0%,Median 0) Among the pre treated patients, plasma EBV DNA copies in stage Ⅲ～Ⅳ patients(Median 3130 0) were higher than those were in stage I～II(Median 400 0), and the copies of N2 3 patients(Median 3250 0) were higher than those were in N0 1 patients(Median 400 0) And then the level of post treated patients(Median 0) was lower than that of pre treated patients(Median 522 0) Depending on the evaluation of therapeutic response, post treated patients could be classified as two different categories, patients whose tumor regressed satisfyingly and those with remaining lesions It was found that EBV DNA copies of the former (Median 0) were lower than those of the latter (Median 2083 0) Conclusions FQ PCR determination of plasma EBV DNA may become a promising marker to monitor the course of this disease
出处
《中华耳鼻咽喉科杂志》
CSCD
北大核心
2004年第3期162-165,共4页
Chinese Journal of Otorhinolaryngology
