摘要
目的 明确脂肪酸合酶 (fattyacidsynthase ,Fas)及其配体 (Fas ligand ,Fas L)在鼻息肉组织的表达及分布 ,并探讨其与鼻息肉发病机制的关系。方法 取 30例鼻息肉组织及同期鼾症手术切除的下鼻甲组织 ,分别液氮保存和 10 %中性甲醛固定 ,应用逆转录聚合酶链反应 (reversetranscriptionpolymerasechainreaction ,RT PCR)和免疫组化方法检测Fas、Fas L在鼻息肉中的表达及分布。结果 基因水平 (RT PCR)Fas L在鼻息肉和下鼻甲组织中表达无明显差异 ,Fas均无明显表达。蛋白水平 (免疫组化 )鼻息肉上皮层及腺体中Fas L的表达 (2 5± 2 1,19± 14 )明显高于下鼻甲组织 (14± 13,12±10 ) ,差异有显著意义 (t=1 6 6 ,P <0 0 1) ;鼻息肉上皮层及腺体中Fas的表达 (13± 10 ,11± 9)明显低于下鼻甲 (17± 11,17± 13) ,差异有显著意义 (t=1 98,P <0 0 1)。结论 Fas、Fas L系统与鼻息肉上皮细胞免疫逃逸、增生 ,腺体不规则囊性扩张及炎性细胞凋亡抑制的发生有关 。
Objectives To confirm the expression and distribution of Fas and Fas L in the nasal polyps and to illustrate the role of the Fas/Fas L system in the pathogenesis of human nasal polyps Methods Investigating the transcripts of the Fas/Fas L gene in 30 human nasal polyp tissues and 30 nasal turbinate mucosa specimens using reverse transcription polymerase chain reaction Localization of Fas/Fas L was performed with immunohistochemistry Results The transcripts of the Fas/Fas L gene were detected at similar levels in both polyps and nasal mucosa There was a significant overexpression of Fas L protein on nasal polyps (epithelium: 25±21, glands: 19±14) compared to nasal mucosa (epithelium: 14±13, glands: 12±10), ( t =1 66, P <0 01), while Fas was overexpressed on nasal mucosa (epithelium: 17±11, glands: 17±13) compared to nasal polyps (epithelium: 13±10, glands: 11±9), ( t =1 98, P <0 01) Fas L positive cells were localized on the epithelial layers of cystically dilated glands and the down growing epithelium of nasal polyps Fas positive cells were localized on the cilia of the epithelial of nasal mucosa and mainly on the infiltrative cells Conclusion Fas/Fas L may play an important role on the pathogenesis of human nasal polyps, including cystic degeneration of submucosal glands, apoptosis and conferring of immune privilege to nasal polyp formation
出处
《中华耳鼻咽喉科杂志》
CAS
CSCD
北大核心
2004年第3期143-146,共4页
Chinese Journal of Otorhinolaryngology
关键词
脂肪酸合酶
配体
鼻息肉
发病机制
逆转录聚合酶链反应
Fatty acid synthase complex
Nasal polyps
Reverse transcription polymerase chain reaction
