摘要
目的观察枳术汤对脾虚便秘小鼠结肠粘膜肥大细胞(MC)的影响及其与P物质(SP)、生长抑素(SS)免疫反应阳性的相关性,探讨该方治疗脾虚便秘的可能作用机制。方法采用饥饱失常和过度疲劳配合燥结便秘的方法复制小鼠脾虚便秘模型,甲苯胺蓝改良法及LSAB免疫组织化学标记法进行MC和SP、SS的检测,用IMS型彩色图像系统和免疫组化分析软件进行分析。结果脾虚便秘模型小鼠结肠粘膜肥大细胞密度明显降低;其MC密度与结肠粘膜SP免疫反应阳性强度均值(r=0.6508,P<0.01)、面积(r=0.684,P<0.01)呈正相关;而与SS免疫反应阳性强度均值(r=-0.7568,P<0.01)、面积(r=-0.683,P<0.01)呈负相关。大、中剂量的枳术汤能使脾虚便秘小鼠结肠粘膜MC密度增加,并一定程度上使SP免疫反应阳性增强,SS的免疫反应阳性减弱。结论调节肠壁粘膜SP、SS免疫反应阳性强度,影响MC释放5-HT、组胺从而调整胃肠运动功能,这可能是枳术汤治疗脾虚便秘的机制之一。
Objective Effect of Zhizhu decoction ( ZD) on mast cells( MC) of colonic mucosa in mice with constipation due to spleen asthenia was observed and the relationship between MC and the immunoreactivity of substance P ( SP) and somatostatin ( SS) was explored to investigate the therapeutic mechanism of ZD.Methods The mice models with constipation due to spleen asthenia were induced by the discordance between starvation and engorgement plus overfatigue. The MC count and SP and SS expression were detected by modified toluidine blue method and LSAB immunohistochemical method respectively, and then analyzed with IMS color image processing system and related software. Results The MC density in the colonic mucosa in the constipated mice was reduced. The MC density was correlated positively with the mean intensity and area of SP immunoreactivity ( r=0.6508, P < 0.01; r=0.684, P < 0.01) and negatively with those of SS immunoreactivity ( r=- 0.7568, P < 0.01; r=- 0.683, P < 0.01) in the colonic mucosa. ZD in high or moderate dose could increase the MC density and the immunoreactivity of SP and decrease the immunoreactivity of SS. Conclusion The therapeutic mechanism of ZD for constipation due to spleen asthenia might be involved in the modulation of gastrointestinal motility via regulating the immunoreactive intensity of SP and SS and then influencing the release of 5- HT and histamine.
出处
《中药新药与临床药理》
CAS
CSCD
2004年第3期167-170,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省中医药管理局资助项目(编号400015)。
关键词
便秘
枳术汤
肥大细胞
免疫
Constipation
Zhizhu decoction
Mast cell
Immunity
