摘要
目的 观察骨髓基质细胞 (marrowstromalcell,MSC)经门静脉移植后在同种异体大鼠体内的转归 ,为MSC体内诱导转化及功能发挥的研究提供基础。方法 采用连续传代培养的方法纯化MSC ,DAPI标记后经门静脉移植入受体 ,移植细胞数量为 10 5个 /只 ,2 0只受体大鼠分为 4组 ,各组分别于移植后 2h、1周、2周、3周、4周处死 ,观察受体肝脏及肺脏内DAPI标记的移植细胞的分布和存活情况。结果 体外培养的MSC具有良好的增殖能力 ,2 0只受体大鼠 4只死亡 ,其余均存活至处死前 ,MSC经门静脉植入异体大鼠后 2h、1周、2周、3周、4周受体肝脏内均可见DAPI标记的移植细胞存活 ,而各期受体肺内未见DAPI标记的移植细胞存活。结论 经门静脉注入MSC后 ,细胞主要分布于受体肝脏 ,在肝脏内分布呈现由门静脉小分支逐渐向肝实质内移行的过程 ,并与肝细胞紧密结合呈索状排列。
Objective To observe the tracing and evolution of marrow stromal cell (MSC) after intraportal transplantation into the liver of homogenous rats,and to provide experimental data for MSC differentiation to hepatocyte in vivo.Methods The MSC was isolated from the leg bone marrow of adult SD rats,and purified by culture-expanded in vitro.Before transplantation,MSC was labeled with DAPI.Then 10 5 MSC were intraportally transplanted into the homogenous rat liver.Rats were killed at 2 hours and 1,2,3,and 4 weeks after transplantation.The cryosection samples of liver and lung were observed under fluorescence microscopy.Results MSC in vitro culture had high ability of proliferation.Except 4 rats were dead because of abdominal bleeding or infection,other recipients were healthy until sacrificed.The implantation cells were detected by identifying the DAPI labeled MSC in the host livers,but not in the host lungs. Conclusion Intraportal transplanted MSC could immigrate and survive in the host livers at least for 4 weeks.They could immigrate from the small branches of portal veins to hepatic parenchyma.
出处
《中华放射学杂志》
CAS
CSCD
北大核心
2004年第2期129-132,共4页
Chinese Journal of Radiology
