摘要
目的 :研究大鼠全脑缺血再灌注损伤后脑内肥大细胞的变化。方法 :采用大鼠 4血管结扎法建立全脑缺血再灌注损伤的模型 ,在手术不同时间后予以心脏灌流、取脑、冰冻切片、甲苯胺兰染色 ,观察肥大细胞的形态并记数。结果 :肥大细胞主要位于丘脑。脑缺血再灌注损伤后 1h、7d,丘脑内肥大细胞数量出现 2次明显的下降 ,而肥大细胞脱颗粒的比例出现 2次明显增加。结论 :脑内肥大细胞在大鼠全脑缺血再灌注损伤后出现明显的脱颗粒 。
Objective:To investigate changes of brain mast cells after transient global ischemia in rats. Methods :Transient global ischemia damage was induced by four-vessel occlusion. After 1 h to 14 days of ischemia, rats were perfused intracardially by 4% paraformaldehyde. The brains were dissected to serial sections using freeze microtome, and then stained with toluidine blue. Brain mast cell was observed under microscope. Results: Most brain mast cells were located in thalamus. The number of mast cells in thalamus markedly decreased during reperfusion after transient global ischemia. However, the degranulation rate of thalamus mast cells showed reverse change after ischemia. Conclusion: Brain mast cells markedly degranulate after transient global ischemia, which may be involved in the pathological process after ischemia.
出处
《浙江大学学报（医学版）》
CAS
CSCD
2004年第3期193-196,200,共5页
Journal of Zhejiang University(Medical Sciences)
基金
国家自然科学基金资助项目 ( No30 3716 38)
浙江省青年人才基金 ( R30 3779)资助项目
