摘要
目的 探讨针刺对缺血再灌注大鼠海马内脑源性神经营养因子 ( BDNF)基因表达的影响 ,推测针刺改善缺血再灌注的可能机制。方法 采用 4-血管阻断法制备大鼠全脑缺血再灌注模型 ,电针刺激百会、肾俞、足三里穴后 ,利用 RT-PCR检测 BDNF m RNA。结果 正常组大鼠海马 BDNF m RNA表达极低 ;缺血再灌注组大鼠海马 BDNF m RNA表达明显增高 ,治疗 1 5 d的针刺 1、2组大鼠海马 BDNF m RNA表达较缺血再灌注组更高 ,及早治疗且治疗时间为 2 0 d的针刺 3组大鼠海马 BDNF m RNA表达降低。结论 缺血再灌注大鼠海马BDNF水平增高有利于损伤的神经元存活、恢复 ;针刺促进脑内细胞分泌内源性 BDNF可能是针刺有效治疗缺血再灌注的机制之一。
Objective To study the change of the brain-derived neurotrophic factor mRNA(BDNF mRNA) in hippocampus of transient focal brain ischemia and reperfusion of rat after by acupuncture therapy. Methods The transient focal brain ischemia and reperfusion model rat was performed by 4-vessel occlusion method. The study chose three acupuncture points Baihui, Shenyu and the Zusanli to treat transient focal brain ischemia and reperfusion rat. The level of BDNF mRNA in rat hippocampus was measured using reverse transcription PCR. Results The level of the BDNF mRNA in hippocampus was very low in the normal rat. But it increased in transient focal brain ischemia and reperfusion model rat. And after acupuncture treatment, it had a significant increase. An earlier begining of therapy which should last long enough for 20 days could make BDNF and mRNA expressions decreased. Conclusions Our demonstration of acupuncture increasing secretin of high level of endogenous BDNF in hippocampus which favoures the survival of injured neuron cells may serve to explain the mechanism of the effective treatment of acupuncture for brain ischemua and reperfusion.
出处
《中国神经免疫学和神经病学杂志》
CAS
2004年第2期98-101,共4页
Chinese Journal of Neuroimmunology and Neurology
基金
贵州省教委 2 0 0 0年度科研基金资助项目 (黔教科 2 0 0 0 15 )
