摘要
研究了山杜英[Elaeocarpus sylvestris(Lour.)Poir.]带芽茎段的离体培养及植株再生。筛选出最佳培养基:(1)启动培养:MS+BA1.0-2.0 mg·L-1(单位下同)+NAA 0.05+蔗糖3％;(2)愈伤组织发生型丛生苗增殖培养:MS+BA 1.0+NAA 0.5+蔗糖3％;腋芽发生型丛生苗增殖培养:MS+BA2.0+IBA 0.1+蔗糖3％;(3)有效苗诱导培养:MS+BA 0.5+IBA 0.1+GA 1.0+蔗糖3％;(4)生根培养:1/2 MS+IBA 3.0+蔗糖2％。用透气膜封口比用聚乙烯菌膜生根率明显提高,生根明显提前。
This paper mainly dealt with the study on shoot organogensis culture in vitro and plant regeneration from sprout explants of Elaeocarpus sylvestris ( Lour. ) Poir. The results showed that the best media for various stages were as follows: (1) Initial medium: MS + BA 1.0 -2. 0 mg ·L-1 + NAA 0. 05 mg · L-1 +3% sucrose; (2) Clump shoot regeneration medium from callus proliferation; MS + BA 1. 0 mg · L-1 + NAA 0. 5 mg · L-1 +3% sucrose; Clump shoot regeneration medium from axillary buds: MS + BA 2.0 mg · L-1 + IB A 0. 1 mg · L-1 + 3% sucrose; (3) Efficiency seedling induction medium; MS + BA 0.5 mg · L-1 +IBA0. 1 mg · L-1 +GA 1.0 mg · L-1 +3% sucrose; (4) Rooting medium: 1/2 MS + IBA 3. 0 mg · L-1 +2% sucrose. In root induction culture, the rate of root formation with membrane filter is higher than that of root formation with common membrane.
出处
《园艺学报》
CAS
CSCD
北大核心
2004年第2期245-248,共4页
Acta Horticulturae Sinica
基金
国务院三峡工程建设委员会移民开发局资助项目(2000-020)
