摘要
[目的]探索金边北海道黄杨再生体系建立的方法和条件。[方法]以MS为基本培养基,配置不同种类和不同浓度激素的培养基,对金边北海道黄杨幼嫩叶片和腋芽进行不定芽诱导培养、增殖培养和生根培养。[结果]MS可作为金边北海道黄杨再生体系的基本培养基。6-BA配合NAA有利于叶片、腋芽愈伤组织和不定芽的诱导,以MS+6-BA1.0 mg/L+NAA0.1 mg/L培养基效果最佳,腋芽诱导率高达66.67%。较高浓度的6-BA配合较低浓度的NAA有利于丛生芽的增殖,以MS+6-BA1.0 mg/L+NAA0.2 mg/L培养基增殖效果较理想,增殖率高达328.57%。MS+IAA0.5 mg/L+蔗糖3%或1/4MS+IAA0.5 mg/L+蔗糖2%可获得最佳生根效果。[结论]该体系的建立为金边北海道黄杨种苗快速繁殖提供参考依据。
[Objective] The study aimed to explore the method and condition for establishing the regeneration system of Euonymus japonicus cv CuZhi.[Method] With MS as basic medium,the tender leaves and axillary buds of CuZhi as materials,the media added 6-BA and NAA with different concentration proportions and the media added IAA with different concn.were set up to conduct the cultures of inducing adventitious buds,proliferation and rooting.[Result] MS could be as basic medium for the regeneration system of CuZhi.The combination of 6-BA and IAA was in favor of inducing calli and adventitious buds from leaves and axillary buds,the effect of medium MS＋6-BA 1.0 mg/L＋NAA 0.1 mg/L was best and the induction rate of axillary buds was up to 66.67%.The combination of 6-BA with higher concentration and NAA with lower concentration was in favor of the proliferation of clustered buds,the proliferation effect of medium MS＋6-BA 1.0 mg/L＋NAA 0.2 mg/L was more perfect and the proliferation rate was up to 328.57%.The optimum rooting
effect could be obtained from medium MS ＋ IAA 0.5 mg/L ＋ 3% sucrose or 1/4MS ＋ IAA 0.5 mg/L ＋ 2% sucrose.[Conclusion] The establishment of the system provided reference basis for the rapid propagation of CuZhi seedlings.
出处
《安徽农业科学》
CAS
北大核心
2008年第6期2245-2246,2346,共3页
Journal of Anhui Agricultural Sciences
基金
河南省科技攻关项目(0524050005)
关键词
北海道黄杨
愈伤组织
再生体系
快繁
Euonymus japonicus cv.CuZhi
Callus
Regeneration system
Rapid propagation
