摘要
从大豆胚轴提取总糖苷后,采用C18反相柱层析法分离大豆异黄酮糖苷,再将其水解制备成异黄酮苷元。产品经薄层色谱法(TLC)和高效液相色谱法(HPLC)分析异黄酮苷元纯度和含量。
Total glycosides were extracted from soybean hypocotyl ,Where isoflavone glycosides were isolated with C18reversed phase column chromatography. Finally isoflavone aglycones were prepared by hydrolysis of isoflavone glycosides.The purity and contents of isoflavone aglycones were assayed with thin layer chromatograghy (TLC) and high-performance liquidchromatography (HPLC).
出处
《食品科学》
EI
CAS
CSCD
北大核心
2004年第4期136-138,共3页
Food Science
基金
国家自然科学基金资助项目(30360113)