摘要
ABC-ELISA方法测定了43例肝癌及8例肝良性占位性病变患者外周血单个核细胞(PBMC)FcγR,并对其中12例肝癌切除术后病人追踪测定。用rIFNγ刺激PBMC,观察FcγR表达量的变化。结果,肝癌组FcγR水平明显低于肝脏良性占位性病变和正常对照组,而后两组比较相差不显著。手术后病人FcγR表达水平提高。肝癌病人内源性IFNγ产生缺陷。外源性IFNγ刺激后FcγR表达显著增强,但仍低于正常对照组。上述结果提示:1.FcγR对鉴别肝脏良恶性占位性病变有参考价值;2.肝癌者FcγR水平低下可能与癌组织分泌某种抑制因子及内源性IFNγ产生低下有关;3.外源性IFNγ促进FcγR表达,是其抗肿瘤机制之一。
The level of FcγR on peripheral blood mononuclear cells (PBMC) was determinedby ABC-ELISA in 43 patients with primary hepatocellular carcinoma (PHC) and in 8patients with space-occupying benign lesion (BL) in liver, twelve patients who wereoperated for PHC were followed up to detect their FcγR. Nine PBMC samples fromPHC were treated with rIFNγ. The results showed that: 1. the level of FcγR in PHCpatients was significantly lower than that in BL and in normal control;2. the expres-sion of FcγR was enhanced in postoperated PHC patients, 3. the production of IFNγin PHC patients was defective. The level of FcγR on PBMC was increased obviouslyin PHC after PBMC being treated with rIFNγ but was still lower than that in control.These results suggested: 1. FcγR dotection is helpful for distinguishing malignantfrom benign; (2) The inhibited expression of FcγR in PHC could be related to somefactors secreted by cancer tissues and also to low level of IFNγ; (3) IFNγ stimulatesthe expression of FcγR in vitro as one of the antitumour mechanism of IFNγ.
出处
《肿瘤》
CAS
CSCD
北大核心
1992年第3期105-107,共3页
Tumor
