摘要
由于FcγRⅢa的基因多态性可导致NK细胞上Fcγ受体(Fcγ receptor,FcγR)与抗肿瘤单克隆抗体(mono-clonal antibody,MAb)Fc段结合的亲和力不同,从而影响NK细胞以抗体依赖细胞介导的细胞毒性(antibody-dependent cell-mediated cytotoxicity,ADCC)对肿瘤细胞的杀伤能力。本研究通过巢式PCR(nest-PCR)扩增43例健康儿童及20例血液肿瘤患儿的FcγRⅢa基因,NlaⅢ酶切PCR扩增产物,酶切后产物经15%PAGE电泳分离DNA片段,银染显色鉴定FcγRⅢa基因多态性;检测健康儿童及血液肿瘤患儿中FcγRⅢa的基因多态性的分布,了解血液肿瘤患儿对MAb可能的疗效反应。结果表明:两组均以FcγRⅢa-158V/F型最多,而FcγRⅢa-158V/V型次之,FcγRⅢa-158F/F型最少,FcγRⅢa的不同基因型及等位基因频率在健康儿童及血液肿瘤患儿中的分布比例基本一致,无明显差异,两组基因型及等位基因频率卡方检验的P值分别为0.809及0.875,均大于0.05。结论:FcγRⅢa-158基因多态性以FcγRⅢa-158V/F型为主,而FcγRⅢa-158V/V型次之;在健康儿童及血液肿瘤患儿中分布无明显差异。
Fcγ, receptor Ⅲa （ FcγR Ⅲ a） polymorphism was considered to influence clinical response to therapeutic monoclonal antibody（MAb） against cancer, which is suggested to affect MAb binding and MAb-dependent NK cell-mediated cytotoxicity. The purpose of this study was to examine the FcγRⅢa gene polymorphisms in healthy children and in children with hematological malignancy, and to explore its possible effect on MAb in children with hematological malignancies. 43 healthy children （H） and 20 pediatric patients with hematological malignancies （HM） were enrolled in this study. DNA was isolated from peripheral blood, and then nest-polymerase chain reaction-restriction fragment length polymorphism （nest-PCR and PCR-RFLP） was used to determine the FcγRⅢ a-158 genotypes in each groups of subject, digested fragments were subjected to electrophoresis on 15% PAGE. The results showed that there were a higher frequencies of FcγRⅢa-158V/F in H and HM group （72. 1% and 75.0% respectively）, the frequencies of FcγR Ⅲ a- 158V/V were 27.9% and 25.0% in H and HM group respectively, but there was no FcγR Ⅲa-158F/F in the two groups. No significant difference in distribution of the FcγRⅢ a-158 genotype was found between HM and H groups （p 〉0.05 ）. It is concluded that Fc-/RⅢa-158V/F is more frequent, while Fc＇,/RⅢa-158V/V is less, but FcγRⅢ a-158F/ F is very rare in both groups. No significant difference of FcγRⅢa polymorphism distribution is found between healthy and hematological malignancy groups.
出处
《中国实验血液学杂志》
CAS
CSCD
2010年第4期959-962,共4页
Journal of Experimental Hematology
基金
留学回国人员科研启动基金(2009年第26批)
