摘要
目的 探讨胆固醇合成抑制剂洛伐他汀 (LOV)对高表达BRCA1基因MCF 7乳腺癌细胞周期素依赖性蛋白激酶抑制剂p2 1的影响。方法 应用基因转染技术获得BRCA1高表达的MCF 7乳腺癌细胞 (MCF 7BRCA1)。 8μmol/LLOV处理细胞 1、2、3d后 ,通过MTT、Western blot等方法检测MCF 7及MCF 7BRCA1两种细胞的增殖功能以及周期素依赖性蛋白激酶抑制剂p2 1表达的改变。结果 LOV处理后 ,细胞生长减慢 ,细胞的增殖能力降低 ,而p2 1的蛋白表达增加 ,其中 ,以BRCA1基因转染的细胞经LOV处理后变化更为显著。结论 BRCA1基因能增强LOV对MCF 7乳腺癌细胞的增殖抑制作用 ,诱导周期素依赖性蛋白激酶抑制剂p2 1活性增加 ,推测这可能是LOV抑制BRCA1基因高表达MCF 7乳腺癌细胞增殖的重要分子机制之一。
Objective To investigate the effects of synthetic inhibitor of cholesterol lovastatin (LOV) on p21 in BRCA1 overexpressing MCF 7 cells. Methods MCF 7 cells were transfected with BRCA1 gene to establish BRCA1 overexpressing MCF 7 cells (MCF 7BRCA1) by gene transfection technigue. After treatment of MCF 7 cells with 8 μmol/L LOV for 1, 2, and 3 d, the cell proliferation capacity of MCR 7 and MCR 7BRCA1 was observed by MTT, and the protein expression of p21 was determined by Western blotting. Results After treatment of MCF 7 cells with LOV, the proliferation capacity decreased, but protein expression of p21 was up regulated. These changes were more significant in BRCA1 transfected cells than the nontransfected cells. Conclusion BRCA1 gene can enhance the inhibitory effect of LOV on the cell proliferation of MCF 7 cells and can induce the up regulated expression of p21, which might be one of the important molecular mechanisms of the inhibitory effect of LOV on the cell proliferation of BRCA1 overexpressing MCF 7 cells.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2004年第5期386-389,共4页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目 ( 30 2 7112 8)~~
