摘要
目的:探讨乙型肝炎病毒(HBV)基因组是否存在前-X编码基因,并探讨前-X基因与HBV基因型之间的关系. 方法:自慢性乙型肝炎患者血清中提取乙型肝炎病毒基因组,首先应用多引物-多聚酶链反应(PCR)进行HBV基因型分型.之后将前-X区扩增,TA克隆到pGEM Teasy载体后进行单克隆测序,利用Vector 8.0软件进行序列分析. 结果:17例患者中,A型者1例,B型3例,B/C混合型者3例,C型10例.克隆测序的45个克隆中,27个(60%) 克隆编码前-X多肽,其中18个(66.7%)克隆来自C基因型.有3种形式突变导致前-X区编码不能.前-X区多肽具有个体化突变现象. 结论:前-X区编码在B、C基因型中均为普遍存在的现象,其编码具有C型倾向性.
AIM: To identify pre-X region in hepatitis B virus (HBV) genome and to study the relationship between the genotype and the existence of pre-X region. METHODS: HBV genomic DNA was extracted from the sera of patients with HBV infection. Multiple-primers polymerase chain reaction (PCR) method was used to identify the genotype of HBV strains. Pre-X region was amplified by PCR method, and TA cloning technique was used to clone the DNA fragment into pGEM Teasy vector. After sequencing of the target region, Vector 8.0 software was used to analysis the sequences. RESULTS: Seventeen samples were collected in this study. One genotype A, 3 genotype B, 10 genotype C and 3 B/C genotype mixture were identified. After sequencing, 27 clones from 45 clones (60%) were found encoding the pre-X peptide. Eighteen from 27 clones (66.7%) pre-X coding sequences were found from C genotype. Three types of replacement mutation led to pre-mature coding of pre-X gene. The mutation in pre-X peptide had feature of individually mutation. CONCLUSION: Coding of the pre-X gene is popular in HBV genome.
出处
《世界华人消化杂志》
CAS
2004年第4期794-800,共7页
World Chinese Journal of Digestology
基金
国家自然科学基金项目
No.C03011402
No.C30070689军队"九
五"科技攻关项目
No.98D063军队回国留学人员启动基金项目
No.98H038军队"十
五"科技攻关青年基金项目
No.01Q138军队"十
五"科技攻关面上项目
No.01MB135~~
