摘要
目的 本实验探讨人鼻息肉组织中嗜酸粒细胞凋亡基因的表达以及红霉素干预对其表达的影响。方法 采用免疫组化方法检测 4 0例离体人鼻息肉组织中嗜酸粒细胞凋亡基因的表达 ,及15例经红霉素干预后 (外植块培养 )的离体人鼻息肉组织中嗜酸粒细胞凋亡基因表达的变化。结果 鼻息肉组织Bcl 2表达 5 % (2 / 4 0 ) ,Bax表达 6 5 % (2 6 / 4 0 ) ,Bcl xL 表达 4 0 % (16 / 4 0 ) ,Fas表达90 % (36 / 4 0 )。与下鼻甲黏膜相比 ,Bax和Fas表达差异有显著性 (P <0 0 5 )。经红霉素干预后 ,鼻息肉组织Bax表达明显上调 (93 3% ,14 / 15 ) ,与单纯用RPMI16 4 0培养者比较差异有显著性 (P <0 0 5 ) ,但Bcl xL和Fas表达差异无显著性。结论 Bax可能是鼻息肉组织嗜酸粒细胞凋亡抑制机制中最有意义的调控基因 ,红霉素可增强Bax的表达 ,是促进嗜酸粒细胞凋亡因素之一。
Objective To study the expression of apoptotic gene in nasal polyps and the possible effect of erythromycin on it. Methods Total 40 nasal polyps biopsies were detected the expression of Bcl-2、Bax、Bcl-x L as well as Fas protein on eosinophils by immunohistochemistry. The expression of Bax、Bcl-x L、Fas on eosinophils in 15 nasal polyps explants which cultured with or without erythromycin were detected also. Results In nasal polyps,the expression of Bcl-2,Bax, Bcl-x L and Fas protein on eosinophils are 5%(2/10), 65%(26/40),40%(16/40) and 90%(36/40) respectively. As compared with inferior turbinate,there were significant difference in expression of Bax and Fas protein between two tissues( P <0.05).After cultured with erythromycin, the expression of Bax protein increased( P <0.05), but the expression of Bcl-x L and Fas protein had not changed . Conclusion Bax may be the most significant gene which control the apoptosis of eosinophils in nasal polyps. And a possible mechaism which erythromycin promote the apoptosis of eosinophils is increase the expression of Bax.
出处
《中华耳鼻咽喉科杂志》
CSCD
北大核心
2003年第6期451-454,I006,共5页
Chinese Journal of Otorhinolaryngology
基金
广东省自然科学基金资助项目 (97 A3 5 7)
广东省医学科研基金项目 (A2 0 0 12 0 4)
