摘要
应用聚丙烯酰胺凝胶电泳技术,对盘鲍(Haliotisdiscus discus )和皱纹盘鲍(Haliotisdis-cushannai)的养殖群体进行了10种等位酶的电泳检测和谱带遗传分析。确定了盘鲍的18个等位酶位点及21个等位基因,单态位点有15个; 仅有3个位点是多态的, 它们是Sod-1,Est-3, -1(P0.99标准),多态位点的百分数为16.67%, 这些多态位点分析到2个等位基因。确 Mdh定了皱纹盘鲍的18个等位酶位点及20个等位基因,单态位点有16个; 仅有2个位点是多态的,它们是Sod-1,Est-3(P0.99标准),多态位点的百分数为11.11%,这些多态位点中也分析到2个等位基因。盘鲍和皱纹盘鲍在所有位点中共享大多数常见等位基因, 因此在生化遗传上它们非常相似。该研究揭示了盘鲍和皱纹盘鲍养殖群体等位酶位点及其等位基因带谱的变异式样,为盘鲍和皱纹盘鲍的遗传结构及遗传育种的研究提供了一批等位酶位点及其等位基因的参考图谱。
Biochemical genetics was investigated using the assay of vertical slab polyacrylamide gel electrophoresis in Haliotis discus discus and H. discus hannai. Ten enzymes presumably encoded by 18 allozyme loci and 21 alleles are scored in H. discus discus, the monomorphic loci are Ldh-1,Adh-1,Sod-2,Est-1,Est-2,Mdh-2,Me-1,Idh- 1,Sdh-1,Aat-1,Aat-2,Aat-3,Amy-1,Amy-2,Amy-3; and 3 loci with two alleles are polymorphic being, Sod-1, -3, -1. The percentage of polymorphicloci(P0.99) is 16.67%. And ten enzymes presumably encoded by 18 Est Mdh allozyme loci and 20 alleles are scored in H. discus hannai, the monomorphic loci are Ldh-1,Adh-1,Sod-2,Est-1, Est-2,Mdh-1,Mdh-2,Me-1,Idh-1,Sdh-1,Aat-1,at-2,Aat-3,Amy-1,Amy-2,Amy-3, and 2 loci with two alleles are polymorphic, which are Sod-1,Est-3. The percentage of polymorphicloci(P0.99) is 11.11%. H. discus discus and H. discus hannai shared most of common alleles in all loci, Therefore they are very similar in biochemical genetic. This study revealed the basic genetic diversity and genetic structure of H. discus discus and H. discus hannai and provided comparable diagrams of allozymes for the researchers in this field.
出处
《海洋科学》
CAS
CSCD
北大核心
2004年第4期43-47,75,共6页
Marine Sciences
基金
国家自然科学基金资助项目(30231013)
福建省自然科学基金资助项目(B0110036)
关键词
盘鲍
皱纹盘鲍
等位酶
遗传结构
等位基因
Haliotis discusdiscus
H.discus hannai
allozyme
biochemicalgenetics
