摘要
运用聚丙烯酰胺凝胶电泳对我国主要养殖鲍 (皱纹盘鲍、盘鲍及九孔鲍 ) 1 2种同工酶 (LDH、ME、MDH、GPI、PGM、IDH、IDDH、SOD、EST、ODH、ADH、AAT) 2 6个基因座位进行了分析 .结果表明 :EST、PGM可以作为区分三种 (亚种 )鲍的生化遗传标记 ;在所分析的位点中 ,未发现多态现象 ,表明三种养殖鲍杂合子缺失严重 ,其主要原因可能在于哑基因。
Twelve isoenzymes ( LDH, ME, MDH, GPI, PGM, IDH, IDDH, SOD, EST, ODH, ADH, AAT) extracted from three species of cultured abalones ( Haliotis discus hannai, H. diversicolor supertexta, H. discus discus ) in China were determined by polyacrylamide gel electrophoresis (PAGE), twenty six genic loci were tested but no polymorphic loci was detected. The result shows: 1) EST and PGM can be used as biochemical genetic markers to identify three species of abalone. 2) Among the loci tested, no polymorphic loci was detected, which means that there is serious loss of heterozygotes. The main reason may be null allele, close breeding and darwin selection.
出处
《台湾海峡》
CAS
CSCD
2003年第2期173-179,T002,共8页
Journal of Oceanography In Taiwan Strait
基金
国家高技术研究发展计划 ( 86 3计划 )课题( 2 0 0 1AA6 2 10 80 )
福建省自然科学基金资助项目 ( 982 0 0 0 2 )
关键词
皱纹盘鲍
盘鲍
九孔鲍
聚丙烯酰胺凝胶电泳
生化遗传学
同工酶
海洋水产
marine fishery
biochemical genetics
polyacrylamide gel electrophoresis
Haliotis discus hannai
Haliotis discus discus
Haliotis diversicolor supertexta
isozyme
