摘要
采用聚丙烯酰胺凝胶电泳技术,对广西北海、福建漳港和江苏启东3个西施舌(Coelomactra antiquata(Spengler))自然群体杂合性进行了研究。共检测分析了7个酶,获得12个位点、25个等位基因。其中4个位点是多态的,分别是Sod-1、Mdh-1、Est-1、Est-3,这些位点分别有1~7个等位基因。结果表明,福建漳港群体的Sod-1和Est-1位点、广西北海群体的Sod-1位点符合Hardy-Weinberg平衡(P〉0.05);江苏启东群体的多态位点中所有位点、福建漳港群体的Mdh-1、Est-3位点、广西北海群体的Mdh-1、Est-1、Est-3位点均非常显著偏离Hardy-Weinberg平衡(P〈0.01)。另外,除了江苏启东和福建漳港群体的Est-1位点表现出杂合子缺失(F〉0)外,各群体中每个位点均表现出杂合子过量(F〈0)。结合各群体中每个多态位点的观察杂合度和期望杂合度,认为中国东南沿海西施舌的种质资源状况良好。
Heterozygosity was investigated using an assay of vertical slab polyacrylamide gel electrophoresis in three Coelmomactra antiquate stocks from three sites: Beihai (Guangxi Province), Zhanggang (Fujian Province), Qidong (Jiangsu Province). 7 allozymes were detected, and got 12 allozyme loci, 25 alleles, 4 loci (Sod-1,Mdh-1,Est-1,Est-3) with 1-7 alleles which are polymorphic. The results showed that the genetypic distribution observed at Sod-1, Est-1 in Zhanggang stock and Sod-1 in Beihai stock were corresponded with the Hardy-Wemberg equilibrium (P〉0.05). But other loci in the three stocks were significant deviations from Hardy-Weinberg equilibrium (P〈0.01). There was heterozygote deficiency existed in Est-1 in Qidong and Zhanggang stock (F〉0), and heterozygote excess existed in other loci in the three stocks (F〈0). The observed heterozygosity and expected heterozygosity per polymorphic locus per stock indicated that the germplasm resources of three C. antiquate stocks were good.
出处
《海洋科学》
CAS
CSCD
北大核心
2009年第7期70-74,共5页
Marine Sciences
基金
福建省自然科学基金项目(B0640010)
福建省高等教育新世纪优秀人才支持计划项目(闽教科〔2006〕35号)
福建省高校水产科学技术与食品安全重点实验室基金项目(2007J202)
集美大学创新团队基金项目(2007A001)