摘要
采用垂直聚丙烯酰胺凝胶电泳技术对西施舌(Coelomactra antiquata)闭壳肌、鳃、外套膜、消化腺和足肌5种组织的18种同工酶进行组织特异性分析,并对其中11种同工酶进行了多态性的筛选。结果表明,西施舌同工酶的表达具有明显的组织特异性。消化腺与肌肉组织中的11种酶(ADH、AK、DIA、EST、GPI、IDH、MDH、PGM、SDH、SOD和XDH)可以得到稳定而清晰的酶谱。在11种酶的17个基因位点中有6个位点(ADH、EST-1、MDH-1、PGM、SOD-1和XDH)为多态位点,多态位点比例为35.3%。平均观测杂合度(Ho)和预期杂合度(He)分别为0.360和0.519,平均等位基因数目(Na)为4.40。
Vertical polyacrylamide gel electrophoresis was employed to investigate the tissue-specificities of allozyme and screen the polymorphic loci in the natural populations of xishishe clam, Coelomactra antiquata in Jimo, Shandong Province. Eighiteen enzyme systems were screened preliminarily in 5 tissues including adductor muscle, gill, mantle, digestive gland and foot muscle. The results showed that the screened enzymes displayed remarkable tissue-specificities and 11 enzyme systems （ADH, AK, DIA, EST, IDH, GPI, MDH, PGM, SDH, SOD and XDH） from the muscle or digestive gland could be reliably scored. Eleven enzymes systems were encoded by 17 loci, and 6 of them revealed polymorphism among the tested 31 individuals, i.e., ADH, EST-1, MDH-1, PGM, SOD-1 and XDH. The proportion of polymorphic loci was 35.3%. The average observed and expected heterozygosities were 0.360 and 0.519 respectively and the mean allele number （Na） was 4.40. These six allozyme marker loci would be useful for the assessment of genetic variation and population structure of this species. [Journal of Fishery Sciences of China, 2008, 15 （1） ： 178-182]
出处
《中国水产科学》
CAS
CSCD
北大核心
2008年第1期178-182,共5页
Journal of Fishery Sciences of China
基金
国家高技术研究发展863计划(2006AA10A409)
国家自然科学基金(30571442).
关键词
西施舌
聚丙烯酰胺电泳
同工酶
组织特异性
多态性
Coelomactra antiquata
polyacrylamide gel electrophoresis
allozyme
tissue-specificity
polymorphism
