摘要
目的 :探讨瘢痕形成和成熟过程中组织内的缺氧状况及其影响因素。方法 :采用免疫组化法检测烧伤后肉芽组织、不同时期瘢痕和正常皮肤中缺氧诱导因子 (HIF - 1α)和分化群 (CD34)的表达 ,采用权重方法分别对各组表皮和真皮的HIF - 1α表达结果进行量化 ,并根据CD34染色结果进行血管计数。结果 :随瘢痕时间的增长 ,HIF - 1α表达强度逐渐减弱 ,血管计数逐渐减少 ,真皮细胞密度逐渐降低。结论 :瘢痕中高细胞密度和高细胞活性是形成瘢痕内缺氧环境的重要原因 ,缺氧环境的改善 ,可促进瘢痕成熟 ,HIF -
Objective: To explore the hypoxic status of the tissue in the course of scar formation and the influencing factors. Method: The expressions of HIF-1α and CD34 in burn wound granulation, burn scar of different phases and normal skin were detected by immunohistochemical method. The expressions of HIF-1α in epidermis and dermis were quantified by weight method and microvessel count was done on the basis of the expression of CD34. Result: As the course of scar formation and maturation developed, the expression of HIF-1α weakened, the count of microvessels and the density of dermis cells decreased gradually. Conclusion: High density and high activity of cells are important factors of the formation of hypoxic environment in scar. Amelioration of hypoxia may accelerate scar maturation. HIF-1α may play an important role in hypoxia amelioration.
出处
《中国烧伤创疡杂志》
2004年第1期4-8,共5页
The Chinese Journal of Burns Wounds & Surface Ulcers
