摘要
用酶释放法检测SLE患者PBMC的NK细胞活性,结果患者NK细胞活性显著低于健康对照者,且与疾病活动度呈负相关。患者NK细胞活性低下与类固醇激素治疗有关,与ANA、抗ds-DNA及抗ENA等自身抗体无关。用K_(562)细胞介导的细胞毒试验检测SLE患者PBMC释放NKCF的能力。结果表明,患者NKCF水平显著低于健康对照者,也与疾病活动程度有关,并与其NK细胞活性水平呈正相关。黄芪和昆明山海棠均可直接或通过刺激NKCF释放促进健康人及SLE患者PBMC的NK细胞活性,并与机体的免疫状态有关。
Natural killer (NK) activity of peripheral blood mononuclear cells (PBMC) from 28 patients with systemic lupus erythematosus (SLE) was measured using enzyme-release assay. The SLE patients had significantly decreased NK activity in comparing with normal controls. The levels of NKactivity correlated with disease activity. Pre-incubation of PBMC saparately with Astragalusembranaceus and Tripterygium hypoglaucum or with their mixture considerably stimulated NKcytotoxicity both in SLE patients and healthy donors. The extent of enhancement was dose-dependent and relevant to pre-incubation periods. The release of a soluble natural killer cytotoxicfactor (NKCF) by peripheral blood mononuclear cells was tested by cytotoxicity assay induced inK_(562) cells. Natural killer cytotoxic factor release was significantly lower in SLE patients than incontrols. The levels of natural killer cytotoxic factor were correlated well with NK activities, butcorrelated negatively with clinical activity. Pre-incubated supernatants from peripheral blood mononuclear cells with above-mentioned agents caused much higher percentage of lysis on K_(562)targets than that of without pre-inchbation
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
1992年第11期669-671,共3页
Chinese Journal of Integrated Traditional and Western Medicine
关键词
黄芪
系统性
红斑狼疮
Astragalus membranaceus
Tripterygium hypoglaucum
mononuclear cell
natural killer activity
SLE
