摘要
目的探讨IgA肾病大鼠肾小球系膜细胞的表型转化及中药复方肾络宁的作用。方法SD大鼠随机分为正常组、模型组、肾络宁组、肾炎康复片组;采用牛血清白蛋白和葡萄球菌肠毒素B复合感染的方法建立大鼠IgA肾病模型;常规检测尿红细胞、尿蛋白、血肌酐,光镜、电镜、荧光免疫观察肾组织病理;免疫组织化学染色和计算机图象分析系统观察大鼠肾小球系膜细胞α-平滑肌肌动蛋白(α-SMA)、纤维连接蛋白(FN)、细胞间黏附分子(ICAM-1)的表达情况。结果模型组肾小球系膜区α-SMA、FN、ICAM-1明显阳性表达(P<0.01),系膜区重度IgA沉积,系膜细胞中度增生,基质增多,毛细血管狭窄,血尿及蛋白尿、血肌酐明显增高;肾络宁及肾炎康复片组α-SMA、FN、ICAM-1表达明显减低(P<0.01),IgA轻度沉积,系膜细胞及基质轻度增多,血尿、蛋白尿及血肌酐明显减低。结论IgA肾病大鼠肾小球系膜细胞发生表型转化,中药复方肾络宁可明显抑制IgA肾病大鼠肾小球系膜细胞的表型转化,改善临床及病理变化,肾络宁是防治IgA肾病的有效方药。
Objectives:To investigate transformation of mesangial cell in rats with IgA nephropathy and effect of Chinese materia medica prescription-Shenluoning.Methods:SD rats were divided into normal group,model group,Shenluoning group,and Shenyan Kangfu tablet group;The model of IgA nephropathy of rats were established by co-infection of bovine serum albumins(BSA) and staphyloentero-toxin B (SEB);RBC,BUN,Cr were examined,pathology of nephridial tissue were investigated by optical and electron microscopy and fluorescent immunoassay;Expression of Mesangial Cell-α,α-SMA,FN,ICAM-1 were observed by Immunohistochemistry and computer image analysis.Results:Expression of Mesangial Cell-α,α-SMA,FN,ICAM-1 of model group were positive(P<0.01),and in this group,IgA heavily deposit in mesangial region,intercapillary cells moderately proliferated,matrix increased,and blood capillary stenosis could be seen,RBC,BUN,and content of Cr in blood obviously increased;In Shenluoning group and Shenyan Kangfu tablet group,expression of Mesangial Cell-α,α-SMA,FN,ICAM-1 significantly decreased(P<0.01),deposit of IgA and proliferation of matrix slightly increased,RBC,BUN,content of Cr in blood obviously decreased.Conclusions:Phenotypic transformation of Mesangial cell occur in rats with IgA Nephropathy.Chinese materia medica prescription-Shenluoning can repress the transformation,improve clinical and pathological change,Shenluoning is effective in treating IgA Nephropathy.
出处
《世界中医药》
CAS
2007年第1期41-44,共4页
World Chinese Medicine
基金
国家自然科学基金项目
No.30472129
