摘要
目的 :研究系统性红斑狼疮 (SLE) ,系统性硬皮病(PSS)和皮肌炎 (DM)患者血清sIL 2R和IL 6在治疗前后含量变化的意义 .方法 :用夹心ELISA法及 3H TdR掺入法检测了临床确诊的结缔组织病 6 0例 (SLE ,PSS ,DM各 2 0例 ) ,并选 30例正常人作对照 .从中选取 30例住院结缔组织病患者 (SLE ,PSS ,DM各 10例 )经临床治愈后 ,采用相同方法检测血清中sIL 2R与IL 6水平的变化 .结果 :sIL 2R ,IL 6与正常对照组相比较高水平的存在于SLE ,PSS ,DM患者血清中 [sIL 2R (kU·L-1) :4 6 6± 198,4 2 1± 192 ,4 12± 174vs118± 5 4 ,P <0 .0 1;2 2 .5± 6 .0 ,2 0 .6± 5 .9,18.8± 4 .0vs9.6± 1.8,P <0 .0 1].SLE伴有肾功能损害患者较不伴肾功能损害者明显升高 [sIL 2R (kU·L-1) :5 99± 12 3vs 4 30±79,P <0 .0 5 ;IL 6 (kU·L-1) :2 5 .1± 6 .5vs 18.2± 4 .6 ,P <0 .0 5 ].患者血清中sIL 2R与IL 6水平呈明显的正相关(r =0 .5 86 ,0 .6 4 3,0 .5 75 ,P <0 .0 5 ) .治疗后较治疗前sIL 2R及IL 6活性水平明显降低 (P <0 .0 5 ) ,但仍然高于正常对照组 (P <0 .0 1) .结论 :SLE ,PSS和DM患者血清sIL 2R和IL 6明显升高 ,两者呈正相关 ,而伴肾功明显损伤者升高更明显 ,治疗后则明显降低 .
AIM: To analyze the changes of sIL 2R and IL 6 in sera of patients with systemic lupus erythematosus (SLE), progressive systemic sclerosis (PSS) and dermatomyositis (DM) before and after treatment and to explore their clinical significance. METHODS: Two sandwich ELISA and 3H TdR incorporation assay were used to measure the levels of sIL 2R and IL 6 in sera of 60 patients (20 cases of SLE, PSS and DM, respectively) and 30 normal individuals. Changes of sIL 2R and IL 6 levels in sera of 30 recovered patients were assayed using the same methods. RESULTS: The levels of sIL 2R and IL 6 in sera of SLE, PSS and DM were higher than those in normal control group [sIL 2R (kU·L -1 ): 466+198, 421+192, 412+174 vs 118 +54, P <0.01; 22.5±6.0, 20.6 ±5.9, 18.8±4.0 vs 9.6±1.8, P <0.01]. The sIL 2R and IL 6 in SLE with renal involvement increased more significantly than those without renal involvment [sIL 2R (kU·L -1 ): 5989+123 vs 430+79, P <0.05; IL 6 (kU·L -1 ) 25.1±6.5 vs 18.2± 4.6, P <0.05]. The levels of sIL 2R and IL 6 positively correlated in SLE, PSS and DM patients, with correlation coefficients r = 0.586, 0.643 and 0.575 respectively ( P <0.05). The levels of sIL 2R and IL 6 in sera of patients after treatment decreased compared with those before treatment ( P <0.05), though still higher than those in normal control group. CONCLUSION: The levels of sIL 2R and IL 6 increase significantly and correlate positively in the sera of patients with connective diseases such as SLE, PSS and DM. The sIL 2R and IL 6 increase more significantly in patients of SLE with renal involvement. The activity of sIL 2R and IL 6 decreases after treatment.
出处
《第四军医大学学报》
北大核心
2003年第10期928-930,共3页
Journal of the Fourth Military Medical University
