摘要
应用组织培养技术进行人工诱导丹参多倍体的研究。结果表明:在组织培养条件下添加10～50ppm秋水仙碱,是诱发丹参多倍体的行之有效的方法。通过试管苗根尖染色体显微鉴定,确证所获得的多倍体为同源四倍体。对经3次以上镜检确认为四倍体的试管苗进行了移栽及田间农艺性状初步鉴定和主要化学成分测定。结果表明,所获的四倍体植株均不同程度地表现出多倍体植株的典型特征,主要化学成分含量亦大多高于原植株,可望进一步选育成新品种。
A study of inducing polyploidy of Salvia miltiorrhiza Bunge in the process of tissue culture was made. The results indicated that it was an effective way to add 10—50 ppm colchicine in MS medium in tissue culture for inducing polyploidy. The chromosome of obtained plantlets was determined by microscopic observation. Four lines were autotetraploid plants. After repeated chromosomal determinations for three times, the four lines were transplanted in the fields for identification and observation in agronomic characteristics. The obtained information demonstrated that these lines showed the typical characters of tetraploid plant. The main chemical constituent in root of tetraploidy was significantly higher than that in original plant. It is hopeful to develop new varieties of S. miltiorrhiza.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1992年第4期224-228,共5页
Journal of China Pharmaceutical University
基金
国家医药局科研项目
关键词
丹参
组织培养
多倍体
Salvia miltiorrhiza Bunge
Tissue culture
Polyploidy
