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桔梗同源四倍体株系的鉴定和桔梗皂甙D含量的比较 被引量:19

Identification and Comparison of Platycodin D Contents among Autotetraploid Lines of Platycodon Grandiflorum A.DC
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摘要 对组织培养条件下诱导获得的桔梗 (PlatycodongrandiflorumA .DC)四倍体株系 ,进行了农艺性状的鉴定 ,同时采用HPLC ELSD测定了其桔梗皂甙D的含量。结果表明 :在供试的 2 1个桔梗四倍体株系中 ,18个株系的桔梗皂甙D含量高于二倍体株系 ;9个株系的单株产量高于二倍体株系 ;15个株系的桔梗皂甙D的生产量高于二倍体株系。这一结果为今后进一步选育出产量高。 The autotetraploid lines of Platycodon grandiflorum A.DC induced by tissue culture were studied. The agronomic characteristic were idendified. At the same time, the content of Platycodin D were determined. The results indicated that the contents of Platycodin D in 18 tetrapolid lines were higher than that in control,and the single plant root yields of 9 tetrapolid lines increased greatly comparing with the control. 15 tetraploid lines showed higher productivity of Platycodin D (yield of Platycodin D/plant) than the control. Obtained results indicate the good prospect to further develop excellent varieties with high yields and high content of Platycodin D of Platycodon grandiflorum A.DC.
机构地区 中国药科大学
出处 《药物生物技术》 CAS CSCD 2004年第6期351-355,共5页 Pharmaceutical Biotechnology
关键词 桔梗 同源四倍体 桔梗皂甙D 高效液相色谱蒸发光散射检测器检测法 Platycodon grandiflorum, Autotetraploid,Platycodin D,HPLC-ELSD
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