摘要
目的 通过观察老年多器官功能障碍综合征 (MODSE)的纤维连接蛋白 (FN )在各重要器官组织 (心、脑、肺、肾和肝 )的分布特点 ,探讨 MODSE的内皮损伤特点及凝血功能失调机制。方法 建立盲肠结扎穿孔术 (CL P)的 MODSE模型 ,设立青年多器官功能障碍综合征 (MODS)组及两组相应的假手术对照组 ,分别于术后 6、12、2 4、4 8和 72 h采用链霉素卵白素生物素的免疫组织化学技术 (L SAB)对各重要器官进行研究。结果 MODSE和青年 MODS组 FN表达强度主要为阴性和弱阳性 ,MODSE组比青年 MODS组 FN降低得快且早 ,而老年假手术组 (SOGE组 )也比青年假手术组 (SOG)的 FN表达强度要弱一些 ,各组织一般在0 .5～ 4 8.0 h降低得比较显著。结论 MODSE、MODS和 SOGE均存在不同程度的内皮细胞损伤和凝血功能障碍 ,其中以 MODSE最为严重。
ObjectiveTo study the characteristic of endothelial injuries and the mechanisms of coagulation disorder in multiple organ dysfunction syndrome in elderly rats by observing the distribution and the intensity of fibronectin(FN) expression in important viscerae including the heart,brain,lung,kidney,liver. MethodsFifty Wistar rats(one half of them were 3 months old,another half 20 months old) were anaesthetized and cecal ligation puncture (CLP) was done. They were defined as MODS group and MODSE group. Forty Wistar rats (one half of them 3 months old,another half 20 months old) were anaesthetized and laparotomy was done as sham operation group(SOG) and sham operation group in elderly(SOGE). The heart,brain,lung,liver and kidney were harvested and were examined histologically at 6,12,24,48 or 72 hours after operation. They were also stained immunohistochemically by using antiFN as the first antibody . Results The expression of FN on the surfaces of vascular endothelial cells in hearts, brains,lungs, livers, kidneys was weakly positive or positive in MODSE,MODS or SOGE group,but it was positive or strongly positive in SOG group. The differences were significant (P<0.05). Conclusion The injury of endothelial cells and the disorders of coagulation system are observed in MODSE,MODS,SOGE groups,especially in MODSE.
出处
《中国危重病急救医学》
CAS
CSCD
2004年第4期202-205,T001,共5页
Chinese Critical Care Medicine
基金
卫生部科研基金资助项目 (94110 1)
