摘要
目的探讨硫化氢(H2S)对多器官功能障碍综合征(MODS)大鼠脏器的保护作用。方法SD大鼠40只,随机分为:①正常对照组;②MODS造模12h组;③MODS造模24h组;④硫氢化钠(NaHS)保护12h组;⑤NaHS保护24h组。试剂盒法检测各组大鼠血清中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、血清丙氨酸氨基转移酶(ALT)、肌酸激酶(CK)和肌酐(Cr)的含量,观察各组大鼠心、肝、肺、肾、小肠的病理组织学变化。结果H2S保护组大鼠血清SOD、GSH-Px明显增高(P<0.05),MDA、ALT、CK和Cr的含量明显降低(P<0.05,P<0.01);H2S保护组大鼠心、肝、肺、肾、小肠的器官损伤综合评分较模型组明显改善(P<0.05)。结论H2S具有明显的抗炎和器官保护作用,对大鼠MODS受损脏器具有保护作用。
Objective To investigate the possible protective effects of hydrogen sulfide （H2S） on rats with multiple organ dysfunction syndrome （MODS）. Methods NariS was taken as a donor of H2S. Forty Sprague-Dawley rats were divided into 5 groups with 8 rats in each group： sham group, MODS 12 h model group, MODS 24 h model group, NariS protection 12 h and 24 h groups. The content of superoxidase dismutase （SOD）, malondialdehyde （MDA）, glutathione peroxidase （GSH-Px）, alanimc transaminse （ALT）, creatine kinase （CK） and creatinine （Cr） in serum were measured, respectively. The histopathological changes in the heart, liver, lung, kidney and small intestine were observed. Results The serum content of SOD and GSH-Px was significantly higher in protected H2S groups （P 〈 0.05）, while MDA, ALT, CK and Cr were significantly lower in protected H2S groups （P 〈 0.05, P 〈 0.01）. Organ injury comprehensive scores were significantly higher in MODS groups than in normal groups （P 〈 0.01）; organ injury comprehensive scores were significantly lower in protected H2S groups than in MODS groups （P 〈 0.05）. Conclusion H2S has obvious protective effects on rats with MODS; thus it can protect the MODS-lesioned organs of rats.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2009年第4期482-485,共4页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
西安交通大学2009年度基础与人文社科研究生创新专项基金资助项目~~
关键词
硫化氢
硫氢化钠
多器官功能障碍综合征
大鼠
hydrogen sulfide NariS
multiple organ dysfunction syndrome （MODS） rat
