摘要
目的 比较研究青年与老龄大鼠脑缺血再灌注 (I/R)后超微结构与神经细胞凋亡特征。方法 采用线栓法建立急性局灶性脑缺血再灌注损伤模型 ,观察缺血 3h及再灌注 3、6、12、2 4和 72 h脑组织超微结构变化及细胞凋亡。结果 老龄大鼠脑缺血 3h和 I/R12 h脑梗死面积较青年大鼠增大。随着 I/R时间延长 ,脑组织细胞损伤逐步加重 ,老龄大鼠较青年大鼠严重。细胞凋亡随着 I/R时间延长而明显增加 ,老龄大鼠出现的早、持续时间长。结论 老年脑缺血再灌注脑梗死面积增大、超微结构损伤和细胞凋亡出现得早且严重。
Objective To study ultrastructured change and neuron apoptosis after focal cerebral ischemia/reperfusion(I/R) in the aged rats. Methods The aged SD rats (20-21 months) and the young (4-5 months) were subjected to 3 hours of middle cerebral artery occulsion with the intraluminal filament technique, followed by reperfusion for 3 hours, 6 hours, 12 hours, 24 hours, 72 hours. The ultrastructure of brain, infarct zone and neuron apoptosis were observed. Results The cerebral infarct zone increased in the aged rats with ischemia for 3 hours and I/R for 12 hours compared with those in the young. The brain injury was obviously deteriorated with I/R time longer, which was serious in the aged than that in the young. The neuron apoptosis increase with I/R time longer, and showed earlier and lasted longer in the aged. Conclusion The cerebral injury is serious and neuron apoptosis increase shows early and lasts longer.
出处
《中国危重病急救医学》
CAS
CSCD
2004年第3期151-154,共4页
Chinese Critical Care Medicine
基金
河南省高校创新人才培养基金 (2 0 0 0 10 )
河南省杰出青年基金资助 (0 412 0 0 0 80 0)
关键词
老年
缺血-再灌注
超微结构
细胞凋亡
aged
cerebral ischemia/reperfusion
ultrastructure
apoptosis
