摘要
目的 ]探讨清热化瘀膏治疗静脉输液渗漏所致静脉炎疗效机制中血管内皮因子 (VEGF)的作用。 [方法 ]将健康新西兰兔 2 7只随机分为A组、B组和C组 ,每组9只。采用 50 %葡萄糖行双侧大隐静脉注射 ,使其部分渗漏 ,A组、B组、C组三组分别给予清热化瘀膏外敷、硫酸镁湿敷、生理盐水湿敷 ,通过SDS聚丙烯酰胺凝胶电泳 (SDS -PAGE)和免疫印迹技术 (Western -blot) ,观察各组血管内皮因子(VEGF )表达量的变化过程。 [结果 ]敷贴药物 8h后 ,A组、B组、C组三组所表达的VEGF量无统计学意义 ;敷药 2 4h后 ,A组VEGF表达量明显减少 ,B组、C组无统计学意义 ;敷药48h后 ,VEGF表达量以A组较少 ,但较 2 4h有明显增加 ,而B组和C组表达量无统计学意义。 [结论 ]清热化瘀膏可通过抑制VEGF引起的血管通透性增加 ,阻断静脉炎的进一步发展 ,达到减轻炎性水肿的目的 。
Objective: to probe into the role of vascular endothelial growth facto r (VEGF) in the mechanism of therapeutic effect on Qingrehuayu ointment to treat patients with phlebitis induced by venoclysis extravasation. Method: a total of 27 he althy New Zealand rabbits were selected and divided into group A, B, and C rando mly and equally (9 rabbits each). Partial venoclysis leakage models of all rabbi ts were made by intravenously infusion of 50% glucose via their bilateral great saphenous vein. Then, rabbits of group A, B, C were given Qingrehuayu ointment e xternal applying, magnesium sulfate wet packing, and normal saline wet packing r espectively. Changes of VEGF expression of all three groups rabbits were observe d via SDS-PAGE and Western blot technique. Result: at the time of 8 h after oint ment applying or wet packing, there were no statistical differences among the th ree groups rabbits in terms of their blood VEGF expression. 24 h after treatment , VEGF expression of group A rabbits decreased remarkably than that of group B a nd C rabbits. There was no statistical difference between group B and group C ra bbits in terms of their VEGF expression. At the time of 48 h after treatment, bl ood VEGF expression of group A rabbits were less than that of the other two grou ps. But it was higher than that of group A rabbits at 24 h after treatment. Howe ver, at the time of 48 h after treatment, there was still no statistical differe nce between group B and group C rabbits in terms of their blood VEGF expression levels. Conclusion: Qingrehuayu ointment external applying may stop the further developing of phlebitis via inhibiting VEGF caused increment of vascular permeab ility. So it has the therapeutic effect of abating inflammatory edema. Moreover, it has a dual-direction adjustable role on the pathological procedure of phlebi tis.
出处
《护理研究》
2004年第4期573-574,共2页
Chinese Nursing Research
基金
2000年度广东省中医药局立项科研课题
关键词
输液渗漏
清热化瘀膏
血管内皮因子
venoclysis leakage
Qingrehuayu ointment
vascular end othelial growth factor
