摘要
目的 :观察tranilast对转化生长因子 -β2 (Transforminggwowthfactor -β2 ,TGF -β2 )促进体外培养人眼小梁细胞胶原合成作用的影响。方法 :采用3 H -脯氨酸掺入及液体闪烁测量技术观察 0 μg·ml-1(对照组 )、 12 5 μg·ml-1、 2 5 μg·ml-1和 5 0 μg·ml-1tranilast对3 2ng·ml-1TGF -β2 促进体外培养人眼小梁细胞胶原合成作用的影响。结果 :12 5 μg·ml-1(q′ =4 2 6,P <0 0 5 )tranilast处理组3 H -脯氨酸掺入量为 62 0 3 3± 80 46,与对照组的 817 3 7± 12 4 2 1比较有显著差异 ;2 5 μg·ml-1(q′ =4 81,P <0 0 1)和 5 0 μg·ml-1(q′ =8 62 ,P <0 0 1)tranilast处理组3 H -脯氨酸掺入量为5 94 5 8± 88 13、 418 64± 67 90 ,与对照组比较有极显著差异 ;同时 ,3 H -脯氨酸掺入量随tranilast浓度增加而减少 ,呈现明显的量效关系。结论 :Tranilast明显抑制TGF -β2 对体外培养人眼小梁细胞胶原合成的促进作用。利用tranilast防治原发开角型青光眼的可能性值得作进一步研究。
To investigate the inhibitory effect of Tranilast on collagen synthesis promotion induced by Transforming Growth Factor-β 2 (TGF-β 2) in cultured human trabecular meshwork cells (TMCs). Methods:Collagen synthesis in cultured 3～5 passage human TMCs were divided and examined at 48h after addition of 0 μg/ml (control),12 5 μg/ml,25 μg/ml,50 μg/ml of Tranilast,respectively with 3 2 μg/ml TGF-β 2 into the incubation medium,by 3H-proline incorporation with liquid scintillation technique. Results:In comparison with the control group,12 5 μg/ml (P<0 05),25 μg/ml (P<0 01),50 μg/ml (P<0 01) Tranilast groups significantly inhibited the incorporation of 3H-proline into the cultured human TMCs promoted by TGF-β 2 in a dose-dependent manner. Conclusions:Tranilast has the inhibitory effect on collagen synthesis promotion induced by TGF-β_2 in cultured human TMCs.
出处
《中国实用眼科杂志》
CSCD
北大核心
2003年第7期555-557,共3页
Chinese Journal of Practical Ophthalmology
基金
国家自然科学基金资助项目 (38970 758)
